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. 1989 Nov;86(22):8946–8950. doi: 10.1073/pnas.86.22.8946

Cloning the interleukin 1 receptor from human T cells.

J E Sims 1, R B Acres 1, C E Grubin 1, C J McMahan 1, J M Wignall 1, C J March 1, S K Dower 1
PMCID: PMC298407  PMID: 2530587

Abstract

cDNA clones of the interleukin 1 (IL-1) receptor expressed in a human T-cell clone have been isolated by using a murine IL-1 receptor cDNA as a probe. The human and mouse receptors show a high degree of sequence conservation. Both are integral membrane proteins possessing a single membrane-spanning segment. Similar to the mouse receptor, the human IL-1 receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. When transfected into COS cells, the human IL-1 receptor cDNA clone leads to expression of two different affinity classes of receptors, with Ka values indistinguishable from those determined for IL-1 receptors in the original T-cell clone. An IL-1 receptor expressed in human dermal fibroblasts has also been cloned and sequenced and found to be identical to the IL-1 receptor expressed in T cells.

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Selected References

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