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. 1989 Dec;86(23):9075–9078. doi: 10.1073/pnas.86.23.9075

Fourier-transform mass spectrometry of large molecules by electrospray ionization.

K D Henry 1, E R Williams 1, B H Wang 1, F W McLafferty 1, J Shabanowitz 1, D F Hunt 1
PMCID: PMC298436  PMID: 2594751

Abstract

The multiply charged ions produced by electrospray ionization of peptides of molecular masses up to 29 kDa have been successfully introduced into a Fourier transform mass spectrometer of unique capabilities for tandem mass spectrometry, large ion dissociation, and resolution. Electrospray ionization places an unusually high number of charges on a peptide yielding mass/charge (m/z) values of 600-1500; in this range at normal operating pressures (approximately 10(-9) torr; 1 torr = 133.3 Pa) Fourier-transform mass spectrometry resolving power is greater than 100,000. Although only 10(-7) torr pressure has been obtained with the initial interface, the resulting resolving power of 5000 makes possible the resolution of isotopic peaks of multiply charged ions. Mass measuring accuracies of a few daltons for molecular masses up to 17 kDa have also been achieved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amster I. J., McLafferty F. W., Castro M. E., Russell D. H., Cody R. B., Jr, Ghaderi S. Detection of mass 16 241 ions by Fourier-transform mass spectrometry. Anal Chem. 1986 Feb;58(2):483–485. doi: 10.1021/ac00293a049. [DOI] [PubMed] [Google Scholar]
  2. Chen L., Wang T. C., Ricca T. L., Marshall A. G. Phase-modulated stored waveform inverse Fourier transform excitation for trapped ion mass spectrometry. Anal Chem. 1987 Feb 1;59(3):449–454. doi: 10.1021/ac00130a016. [DOI] [PubMed] [Google Scholar]
  3. Cody R. B., Jr, Amster I. J., McLafferty F. W. Peptide mixture sequencing by tandem Fourier-transform mass spectrometry. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6367–6370. doi: 10.1073/pnas.82.19.6367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Covey T. R., Bonner R. F., Shushan B. I., Henion J. The determination of protein, oligonucleotide and peptide molecular weights by ion-spray mass spectrometry. Rapid Commun Mass Spectrom. 1988 Nov;2(11):249–256. doi: 10.1002/rcm.1290021111. [DOI] [PubMed] [Google Scholar]
  5. Hunt D. F., Shabanowitz J., Yates J. R., 3rd, Zhu N. Z., Russell D. H., Castro M. E. Tandem quadrupole Fourier-transform mass spectrometry of oligopeptides and small proteins. Proc Natl Acad Sci U S A. 1987 Feb;84(3):620–623. doi: 10.1073/pnas.84.3.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hunt D. F., Zhu N. Z., Shabanowitz J. Oligopeptide sequence analysis by collision-activated dissociation of multiply charged ions. Rapid Commun Mass Spectrom. 1989 Apr;3(4):122–124. doi: 10.1002/rcm.1290030408. [DOI] [PubMed] [Google Scholar]
  7. Loo J. A., Udseth H. R., Smith R. D. Peptide and protein analysis by electrospray ionization-mass spectrometry and capillary electrophoresis-mass spectrometry. Anal Biochem. 1989 Jun;179(2):404–412. doi: 10.1016/0003-2697(89)90153-x. [DOI] [PubMed] [Google Scholar]
  8. Wibf H. K., Irving P., McCamish M., Venkataraghavan R., McLafferty F. W. Mass spectrometric studies of peptides. V. Determination of amino acid sequences in peptide mixtures by mass spectrometry. J Am Chem Soc. 1973 May 16;95(10):3369–3375. doi: 10.1021/ja00791a048. [DOI] [PubMed] [Google Scholar]
  9. Williams R. E., Berry J. G. Evaluation of CGA 72662 as a topical spray and feed additive for controlling house flies breeding in chicken manure. Poult Sci. 1980 Oct;59(10):2207–2212. doi: 10.3382/ps.0592207. [DOI] [PubMed] [Google Scholar]

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