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. 1989 Dec;86(23):9119–9123. doi: 10.1073/pnas.86.23.9119

Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.

A Laban 1, D F Wirth 1
PMCID: PMC298445  PMID: 2594753

Abstract

We report a transient expression transfection system in Leishmania enriettii. A hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the alpha-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.

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Selected References

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