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. 1989 Dec;86(23):9159–9162. doi: 10.1073/pnas.86.23.9159

Antibacterial peptides from pig intestine: isolation of a mammalian cecropin.

J Y Lee 1, A Boman 1, C X Sun 1, M Andersson 1, H Jörnvall 1, V Mutt 1, H G Boman 1
PMCID: PMC298453  PMID: 2512577

Abstract

Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatography. Sequence analysis showed the anti-Bm factor to be apparently similar to vasoactive intestinal peptide. The anti-Ec factor was found to have a 31-residue sequence that was cecropin-like. It was named cecropin P1 and its structure was confirmed by solid-phase synthesis. Synthetic cecropin P1 with and without C-terminal amide was assayed on eight different bacteria. Mobility comparison between synthetic and natural cecropin P1 indicates that the natural peptide has a free C-terminal carboxyl group.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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