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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1989 Dec;86(23):9465–9469. doi: 10.1073/pnas.86.23.9465

Blocking of passive sensitization of human mast cells and basophil granulocytes with IgE antibodies by a recombinant human epsilon-chain fragment of 76 amino acids.

B Helm 1, D Kebo 1, D Vercelli 1, M M Glovsky 1, H Gould 1, K Ishizaka 1, R Geha 1, T Ishizaka 1
PMCID: PMC298517  PMID: 2512581

Abstract

The recombinant peptide corresponding to residues 301-376 at the junction of constant regions 2 and 3 of the human IgE epsilon chain blocked the in vivo passive sensitization of human skin mast cells and in vitro sensitization of human basophil granulocytes with human IgE antibodies. An injection of the recombinant peptide or E myeloma protein into normal skin sites 1 hr before sensitization with an allergic serum blocked passive sensitization. In this system, approximately 10-fold higher molar concentration of the recombinant peptide than E myeloma protein was required for 50% inhibition of Prausnitz-Küstner reactions. When the mononuclear cells of two normal individuals were preincubated with the recombinant peptide or E myeloma protein for 15 min before passive sensitization with the same allergic serum and the cells were challenged with an optimal concentration of an antigen, approximately 11- to 13-fold higher concentration of the recombinant peptide than E myeloma protein was required for 50% inhibition of antigen-induced histamine release. Further studies with several recombinant peptides indicated that amino acid resides 363-376 in the Fc epsilon-chain fragment are not essential for binding of the peptide to Fc epsilon-chain receptor I.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coleman J. W., Helm B. A., Stanworth D. R., Gould H. J. Inhibition of mast cell sensitization in vitro by a human immunoglobulin epsilon-chain fragment synthesized in Escherichia coli. Eur J Immunol. 1985 Sep;15(9):966–969. doi: 10.1002/eji.1830150920. [DOI] [PubMed] [Google Scholar]
  2. Ellsworth R. K., Nowak C. A. A gas chromatographic-mass spectrometric analysis of the esterifying alcohols of pumpkin seed protochlorophylls. Anal Biochem. 1974 Feb;57(2):534–546. doi: 10.1016/0003-2697(74)90108-0. [DOI] [PubMed] [Google Scholar]
  3. Geha R. S., Helm B., Gould H. Inhibition of the Prausnitz-Küstner reaction by an immunoglobulin epsilon-chain fragment synthesized in E. coli. Nature. 1985 Jun 13;315(6020):577–578. doi: 10.1038/315577a0. [DOI] [PubMed] [Google Scholar]
  4. Guo L. H., Wu R. Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides. Methods Enzymol. 1983;100:60–96. doi: 10.1016/0076-6879(83)00046-4. [DOI] [PubMed] [Google Scholar]
  5. Helm B., Marsh P., Vercelli D., Padlan E., Gould H., Geha R. The mast cell binding site on human immunoglobulin E. Nature. 1988 Jan 14;331(6152):180–183. doi: 10.1038/331180a0. [DOI] [PubMed] [Google Scholar]
  6. Ishizaka K., Ishizaka T., Lee E. H. Biologic function of the Fc fragments of E myeloma protein. Immunochemistry. 1970 Aug;7(8):687–702. doi: 10.1016/0019-2791(70)90175-8. [DOI] [PubMed] [Google Scholar]
  7. Ishizaka T., Helm B., Hakimi J., Niebyl J., Ishizaka K., Gould H. Biological properties of a recombinant human immunoglobulin epsilon-chain fragment. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8323–8327. doi: 10.1073/pnas.83.21.8323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ishizaka T., Ishizaka K. Biology of immunoglobulin E. Molecular basis of reaginic hypersensitivity. Prog Allergy. 1975;19:60–121. [PubMed] [Google Scholar]
  9. Kenten J. H., Molgaard H. V., Houghton M., Derbyshire R. B., Viney J., Bell L. O., Gould H. J. Cloning and sequence determination of the gene for the human immunoglobulin epsilon chain expressed in a myeloma cell line. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6661–6665. doi: 10.1073/pnas.79.21.6661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kenten J., Helm B., Ishizaka T., Cattini P., Gould H. Properties of a human immunoglobulin epsilon-chain fragment synthesized in Escherichia coli. Proc Natl Acad Sci U S A. 1984 May;81(10):2955–2959. doi: 10.1073/pnas.81.10.2955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pruzansky J. J., Grammer L. C., Patterson R., Roberts M. Dissociation of IgE from receptors on human basophils. I. Enhanced passive sensitization for histamine release. J Immunol. 1983 Oct;131(4):1949–1953. [PubMed] [Google Scholar]
  12. Rosenberg M., Ho Y. S., Shatzman A. The use of pKc30 and its derivatives for controlled expression of genes. Methods Enzymol. 1983;101:123–138. doi: 10.1016/0076-6879(83)01009-5. [DOI] [PubMed] [Google Scholar]
  13. Sussman G. L., Harvey R. P., Schocket A. L. Evaluation of skin test response using two techniques of measurement. Ann Allergy. 1982 Feb;48(2):75–77. [PubMed] [Google Scholar]

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