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. 1989 Dec;86(24):9941–9945. doi: 10.1073/pnas.86.24.9941

An altered v-raf is required in addition to v-myc in J3V1 virus for acceleration of murine plasmacytomagenesis.

J Troppmair 1, M Potter 1, J S Wax 1, U R Rapp 1
PMCID: PMC298618  PMID: 2690079

Abstract

We have isolated and molecularly cloned a highly pathogenic virus variant, J3V1, from murine plasmacytomas induced by a combination of pristane and the weakly transforming recombinant retrovirus J3. J3 virus is a derivative of the v-raf/v-myc-carrying J2 virus that was generated by a frameshifting deletion inactivating v-raf in J2. J3V1 contains an additional deletion of 334 base paris in gag, which restores the correct reading frame for v-raf and results in the expression of a p57 gag-raf fusion protein. Reactivation of v-raf in J3 is required for efficient plasmacytoma acceleration in pristane-conditioned BALB/cAn mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M., Harris A. W., Pinkert C. A., Corcoran L. M., Alexander W. S., Cory S., Palmiter R. D., Brinster R. L. The c-myc oncogene driven by immunoglobulin enhancers induces lymphoid malignancy in transgenic mice. Nature. 1985 Dec 12;318(6046):533–538. doi: 10.1038/318533a0. [DOI] [PubMed] [Google Scholar]
  2. Cleveland J. L., Huleihel M., Bressler P., Siebenlist U., Akiyama L., Eisenman R. N., Rapp U. R. Negative regulation of c-myc transcription involves myc family proteins. Oncogene Res. 1988;3(4):357–375. [PubMed] [Google Scholar]
  3. Clynes R., Wax J., Stanton L. W., Smith-Gill S., Potter M., Marcu K. B. Rapid induction of IgM-secreting murine plasmacytomas by pristane and an immunoglobulin heavy-chain promoter/enhancer-driven c-myc/v-Ha-ras retrovirus. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6067–6071. doi: 10.1073/pnas.85.16.6067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Huleihel M., Goldsborough M., Cleveland J., Gunnell M., Bonner T., Rapp U. R. Characterization of murine A-raf, a new oncogene related to the v-raf oncogene. Mol Cell Biol. 1986 Jul;6(7):2655–2662. doi: 10.1128/mcb.6.7.2655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Morse H. C., 3rd, Hartley J. W., Fredrickson T. N., Yetter R. A., Majumdar C., Cleveland J. L., Rapp U. R. Recombinant murine retroviruses containing avian v-myc induce a wide spectrum of neoplasms in newborn mice. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6868–6872. doi: 10.1073/pnas.83.18.6868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ohno S., Migita S., Wiener F., Babonits M., Klein G., Mushinski J. F., Potter M. Chromosomal translocations activating myc sequences and transduction of v-abl are critical events in the rapid induction of plasmacytomas by pristane and abelson virus. J Exp Med. 1984 Jun 1;159(6):1762–1777. doi: 10.1084/jem.159.6.1762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Potter M., Mushinski J. F., Mushinski E. B., Brust S., Wax J. S., Wiener F., Babonits M., Rapp U. R., Morse H. C., 3rd Avian v-myc replaces chromosomal translocation in murine plasmacytomagenesis. Science. 1987 Feb 13;235(4790):787–789. doi: 10.1126/science.3810165. [DOI] [PubMed] [Google Scholar]
  8. Potter M. Plasmacytomas in mice. Semin Oncol. 1986 Sep;13(3):275–281. [PubMed] [Google Scholar]
  9. Principato M., Klinken S. P., Cleveland J. L., Rapp U. R., Holmes K. L., Pierce J. H., Morse H. C., 3rd In vitro transformation of murine bone marrow cells with a v-raf/v-myc retrovirus yields clonally related mature B cells and macrophages. Curr Top Microbiol Immunol. 1988;141:31–41. doi: 10.1007/978-3-642-74006-0_6. [DOI] [PubMed] [Google Scholar]
  10. Rapp U. R., Cleveland J. L., Fredrickson T. N., Holmes K. L., Morse H. C., 3rd, Jansen H. W., Patschinsky T., Bister K. Rapid induction of hemopoietic neoplasms in newborn mice by a raf(mil)/myc recombinant murine retrovirus. J Virol. 1985 Jul;55(1):23–33. doi: 10.1128/jvi.55.1.23-33.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rapp U. R., Goldsborough M. D., Mark G. E., Bonner T. I., Groffen J., Reynolds F. H., Jr, Stephenson J. R. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4218–4222. doi: 10.1073/pnas.80.14.4218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rapp U. R., Heidecker G., Huleihel M., Cleveland J. L., Choi W. C., Pawson T., Ihle J. N., Anderson W. B. raf family serine/threonine protein kinases in mitogen signal transduction. Cold Spring Harb Symp Quant Biol. 1988;53(Pt 1):173–184. doi: 10.1101/sqb.1988.053.01.023. [DOI] [PubMed] [Google Scholar]
  13. Rapp U. R., Reynolds F. H., Jr, Stephenson J. R. New mammalian transforming retrovirus: demonstration of a polyprotein gene product. J Virol. 1983 Mar;45(3):914–924. doi: 10.1128/jvi.45.3.914-924.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schultz A. M., Copeland T., Oroszlan S., Rapp U. R. Identification and characterization of c-raf phosphoproteins in transformed murine cells. Oncogene. 1988 Feb;2(2):187–193. [PubMed] [Google Scholar]
  15. Stern D. F., Roberts A. B., Roche N. S., Sporn M. B., Weinberg R. A. Differential responsiveness of myc- and ras-transfected cells to growth factors: selective stimulation of myc-transfected cells by epidermal growth factor. Mol Cell Biol. 1986 Mar;6(3):870–877. doi: 10.1128/mcb.6.3.870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sümegi J., Spira J., Bazin H., Szpirer J., Levan G., Klein G. Rat c-myc oncogene is located on chromosome 7 and rearranges in immunocytomas with t(6:7) chromosomal translocation. Nature. 1983 Dec 1;306(5942):497–498. doi: 10.1038/306497a0. [DOI] [PubMed] [Google Scholar]
  17. Troppmair J., Huleihel M., Cleveland J., Mushinski J. F., Kurie J., Morse H. C., 3rd, Wax J. S., Potter M., Rapp U. R. Plasmacytoma induction by J series of v-myc recombinant retroviruses: evidence for the requirement of two (raf and myc) oncogenes for transformation. Curr Top Microbiol Immunol. 1988;141:110–114. doi: 10.1007/978-3-642-74006-0_15. [DOI] [PubMed] [Google Scholar]
  18. van Lohuizen M., Verbeek S., Krimpenfort P., Domen J., Saris C., Radaszkiewicz T., Berns A. Predisposition to lymphomagenesis in pim-1 transgenic mice: cooperation with c-myc and N-myc in murine leukemia virus-induced tumors. Cell. 1989 Feb 24;56(4):673–682. doi: 10.1016/0092-8674(89)90589-8. [DOI] [PubMed] [Google Scholar]

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