Non-vascularized fibular graft reconstruction after resection of giant aneurysmal bone cyst (ABC)

Abstract

The aim of this study was to present the results of non-vascularized fibular graft for reconstruction of bone defects after en block resection of giant aneurysmal bone cyst (ABC) of the extremities. Between 1998 and 2006, three patients, aged 6, 8 and 23 years, with giant aneurysmal bone cysts were treated. The cysts were located in the humerus, proximal femur and metatarsal. All patients were given en bloc resection of the cyst followed by non-vascularized fibular bone graft, with the graft length ranging from 6 to 18 cm. All patients needed supplementary fixation with a single Kirschner wire or plate and screws. At the final follow-up, bony union was achieved in each case, and there was no recurrence, limitation of range of motion or disability. In addition, complete regeneration of the fibula at the donor site was seen in the two children. We propose a criterion for giant ABC, when the transverse diameter of the cyst is up to three times or more of the transverse diameter of the nearby bone, it is then called a giant ABC. Non-vascularized fibular graft is an optimal and valuable method for the reconstruction of bone defects after resection of giant ABC in the extremities.

Introduction

ABC is a benign, expansile, non-neoplastic lesion of the bone, characterized by channels of blood and spaces separated by fibrous septa [1]. Although it is a benign lesion, malignant transformation has been reported [2]. Seventy-five percent of the lesions occur in the first 2 decades of life, and almost 95% occur in the first 3 decades [3]. It has been shown that at least a subset of ABCs has a specific cytogenetic aberration, most commonly a translocation [4]. It was suggested that ABC corresponds to a hemodynamic disturbance because of primary or secondary venous malformation of bones [5]. Giant ABC is an uncommon pathological lesion and can be challenging because of the destructive effect of the cyst on the bones and the pressure on the nearby structures, especially on weight-bearing bones.

En bloc resection of the diseased segment is a useful surgical technique in the treatment of benign and malignant bone lesions. The fibula is a strong, long, tubular bone that can be used as a non-vascularized bone graft for restoration of mechanical continuity and efficiency of the limb after bone defects resulting from tumor resection [6–8]. The purpose of this article is to evaluate the clinical and radiological results of the resection and reconstruction of giant ABCs using non-vascularized fibular bone graft.

Patients and methods

Between 1998 and 2006, three patients with florid manifestations of giant ABC of the extremities were treated with en bloc resection and reconstruction by non-vascularized strut fibular grafts. There were two children aged 6 and 8 years old and one 23-year-old adult; all were males. The cysts were present in the upper third of the humerus, the proximal part of the femur and the metatarsal diaphysis (Table 1). All patients had painful swelling of 6–14 months’ duration. All patients were followed up regularly for 3–8 years (mean 5 years). Imaging studies included conventional radiographs and magnetic resonance imaging (MRI). The length of the required fibula for bridging the bone defect was estimated from the MR scans. The strut fibular graft was harvested after resection of the cyst by a lateral skin incision over the middle of the ipsilateral fibula. After that, exposure of the middle of the fibula was performed, and it was dissected sub-periosteally. Care was taken that more than 5 cm of the proximal and distal fibula was preserved to ensure stability of the knee and ankle. The periosteum was closed in children to facilitate fibular regeneration, and the wound was closed in layers without drainage and then dressed.

Table 1.

Demographic and clinical data of patients

Case no.	Age/sex	Site	Size/cm	TDC/TDB	Type of surgical procedure	Graft length (cm)	Complications	Follow-up (years)
1	8/M	3rd Metatarsal	5 × 6	5/0.7	1st stage: Resection, graft harvesting, insertion of the strut graft and fixation by Kirschner wire. 2nd stage: removal of the Kirschner wire	6	–	4
2	23/M	Proximal third humerus	9 × 10	9/3	1st stage: Open biopsy. 2nd stage: Resection, graft harvesting, insertion of the strut graft, fixation by intramedullary Kirschner wire and circlage wire distally. 3rd stage: Autogenous cancellous bone graft, open reduction and internal fixation by plate and screws for non-union. 4th stage: Removal of the plate and screws	18	Non-union	8
3	6/M	Proximal femur	8 × 8	8/1.5	1st stage: Open biopsy. 2nd stage: Resection, graft harvesting, insertion of the dual strut graft. 3rd stage: Open reduction and internal fixation by plate and screws for delayed union. 4th stage: removal of the plate and screws	10 × 2	Delayed union	3

TDC transverse diameter of the cyst, TDB transverse diameter of the nearby normal bone

Case 1

An 8-year-old male child presented with painful swelling in the dorsum of the right foot of 6 months' duration. Conventional plain radiographs of the foot showed fusiform dilation and ballooning with a radiolucent area of the metatarsal diaphysis with a very thin outline, causing pressure on the surrounding metatarsal bones. MRI revealed an expansile lobulated bone lesion with multiple septations in the diaphysis of the affected bone (Fig. 1). The lesion was hypointense on T1- and hyperintense on T2-weighted images, and the cortices were disrupted. The surgical plan was en bloc excision of the lesion with incorporation of an autogenous interpositional non-vascularized fibular graft. In the operation, a dorsolinear incision was placed over the cystic lesion. The incision passed through layers to the level of the cyst, exposing the full length of the affected bone, which revealed fusiform swelling, as anticipated from plain radiographic and MRI findings. Frozen section indicated the benign nature of the lesion. The thin-walled tumor was resected en bloc with a microsagittal saw. The defect was replaced by a 6-cm segment from the ipsilateral fibula and fixed by Kirschner wire across the osteotomy sites. Postoperatively, the patient used a below-the-knee, non-weight-bearing cast for 6 weeks, then gradually weight bearing was tolerated for another 6 weeks. After 12 weeks, the Kirschner wire was removed, and the patient was allowed a gradual range of joint movement. Radiological follow-up every 3 months in the 1st year then yearly showed complete union with complete graft incorporation and consolidation. There was no evidence of recurrence, as determined radiologically. Moreover, the function of the limb was full, without any disability. Two cases of lesions of the proximal third of the humerus and proximal metaphyseal part of the femur were considered highly aggressive with no defined periosteal shell or bone limits.

Fig. 1.

Case 1: An 8-year-old male child presented with painful swelling in the dorsum of the right foot. a Anterior posterior (AP) plain radiograph of the right foot showing an expansive cystic lesion replacing the entire third metatarsal, except for the proximal and distal epiphyses. Diffuse endosteal thinning without a fracture, leaving the cortex as a thin shell with “a balloon” appearance, caused a pressure effect on the nearby metatarsals. b Transverse T2 MRI views of the right foot showing fluid level and hemorrhage inside the cyst. c Sagittal T2 MRI views of the right foot showing a multilobular pattern and hemorrhage inside the cyst. d AP plain radiograph of the right foot 2 weeks postoperatively showing the fibular graft in situ fixed by Kirshner wire. e AP plain radiograph of the right foot 4 years after surgery showing healing, consolidation, remodeling and preserved length of the metatarsal bone. f Photograph of the right foot at the final follow-up showing the surgical scar and alignment of the toes

Case 2

A 23-year-old male patient presented with pathological humeral fracture to another hospital. He had open biopsy followed by local curretage and a fibular strut graft without internal fixation. After 14 months, the patient presented to our hospital with painful swelling in the left arm. The patient underwent complete investigations to exclude malignant tumor. Plain radiography showed an osteolytic “blow-out” lobulated lesion outlined by a paper-thin shell of cortices. Magnetic resonance image (MRI) demonstrated the presence of a 9.0 × 10.0-cm large cystic expansile lesion in the proximal third of the humerus (Fig. 2). Open biopsy confirmed the benign nature of the aneurysmal bone cyst. Two weeks later, the patient had a wide local resection with a 1.5-cm safety margin as a precaution because of the aggressive nature of the lesion. The defect was bridged by a strut fibular graft that was introduced proximally through a tunnel that was made in the remaining part of the proximal humerus and passed distally through a slit that was made in the remaining distal humerus. Intramedullary, a 3-mm Kirschner wire was introduced through the graft proximally and distally. A cerclage wire was used to hold the fibular graft in the distal part of the humerus. After 18 months, the patient had open reduction and internal fixation with the addition of cancellous iliac crest bone graft for non-union of the distal part of the fibula with the humerus. Careful follow-up showed complete consolidation of the bone, and the patient had a full range of shoulder movement. The plate and screws were removed after 4 years. At 8 years there were hypertrophy and remodeling of the fibular graft.

Fig. 2.

Case 2: A 23-year-old male patient was diagnosed with ABC of the humerus. a Anterior posterior (AP) plain radiograph of the left humerus showing pathological fracture of the shaft of the humerus. b AP plain radiograph of the left humerus showing the pieces of the fibular graft at the site of the fracture. c AP plain radiograph of the left humerus showing a large expansile cystic lesion causing total destruction of the bone with ballooning effect. d Coronal T2 MRI views of the left humerus showing multiple fluid levels with hemorrhage inside the cyst. e AP plain radiograph of the left humerus in the immediate postoperative period showing the graft in situ fixed proximally and distally. f AP plain radiograph of the left humerus after 18 months showing healing in the proximal part of the graft and non-union at the distal part. g AP plain radiograph of the left humerus after open reduction and internal fixation (ORIF) with addition of bone graft. h AP plain radiograph of the left humerus after removal of metal showing incorporation of the graft proximally and distally. i AP plain radiograph of the left humerus 8 years postoperatively showing good consolidation, hypertrophy and remodeling of the graft

Case 3

A 6-year-old male child presented with painful limping on the left side of 12 months' duration. Physical examination demonstrated diffuse swelling in the left hip region with marked tenderness and limitation of the hip range of movement. Plain radiography of the pelvis and hips showed an osteolytic “blow-out” lobulated lesion outlined by a paper-thin shell of cortex over the proximal part of the femur bone. MRI demonstrated the presence of an 8.0 × 8.0-cm multilocular bone cyst involving the neck and proximal part of the femur (Fig. 3). Well-demarcated multiple fluid levels and internal hemorrhage were also noted. After the diagnosis of ABC was ensured by open biopsy, resection of the tumor through peeling the cyst from the outer periosteum was done without damaging the neurovascular bundles or the surrounding attached tendons. The remaining defect in the proximal femur was bridged by two struts of fibular graft (each 10 cm in length) harvested subperiosteally from the middle two thirds of the ipsilateral fibula to allow for future regeneration of the fibula. The remaining periosteal sheath of the cyst was closed over the fibular graft, without using internal fixation. Postoperatively, the patient was kept in hip spica for 2 months, then mobilized on crutches for another 4 months. The patient needed fixation of the graft by plate and screws after he had delayed union 4 months after the initial operation as manifested by angulations of the distal part of the graft with the femur. Postoperatively, the patient used crutches with no weight bearing for another 3 months.

Fig. 3.

Case 3: A 6-year-old male child was diagnosed with ABC of the proximal femur. a Anterior posterior plain radiograph of the pelvis and hips showing an expansile multilocular lytic lesion of the proximal left femur, causing significant destruction with loss of bone architecture. b Coronal T2-weighted MR image shows the bright signals of an expansile, lobulated, cystic mass on the proximal femur, intracystic hemorrhage. c AP plain radiograph of the left hip and proximal femur after 3 years of surgery showing good incorporation of the graft, consolidation of the proximal femur, preservation of the hip joint and loose proximal screws

Removal of the plate and screws took place after 2 years when full incorporation of the graft was achieved proximally and distally. Grossly, all resected specimens were cystic, with several hollow spaces of varying sizes that were filled up with blood clots. Microscopic examination revealed many septae-forming blood-filled channels. Multinucleated giant cells, osteoid and woven bones were apparent in the septae. No malignant cells were found.

Discussion

We could not find the term “giant aneurysmal bone cyst” in the literature. ABC can present early before reaching giant size, which facilitates early diagnosis and treatment. It is well known that ABC is classified as an aggressive benign bone tumor, which means if not treated properly, it may recur, or if left untreated, it may get larger and be called giant ABC. Although the pathogenesis of ABC is still unknown, the two broad types are either primary (70%) or secondary (30%). Primary ABCs arise de novo. A secondary ABC develops in association with other neoplasms, most commonly GCT of the bone, osteoblastoma, chondroblastoma and fibrous dysplasia [3]. Radiographically, the diagnosis of an ABC shows five classic findings [9]. First, the neoplasm typically presents as an expansile lytic lesion with a soap-bubble appearance. Second, it presents an eccentric lesion outlined by a thin layer of subperiosteal new bone. Third, it presents a centric lesion. Fourth, it reveals a metaphyseal lesion that occupies a large percentage of the bone with trabeculations at the edges. Fifth, it manifests soft tissue expansion and destruction of the cortex. None of these criteria could fit any of our patients; consequently, we suggest that, if the cyst's transverse diameter on radiographic examination is equal to or more than three times the diameter of the adjacent normal bone, we can call it giant ABC; in this case, it can be treated by resection and fibular strut graft. Most of the available surgical methods are suitable for treating metaphyseal localized lesions. However, with the use of extensive curettage and bone graft with or without adjuvant methods, there is a considerably high rate of complications [10, 11]. However, when the extremity lesion is giant, any treatment method other than complete resection and reconstruction will have a high propensity to increased morbidity of repeated surgeries and a high possibility for pathological fracture. In giant ABCs, preservation of the bone length is important for good function of the affected limb and nearby joints. In our patients, none of the common modalities of treatment other than complete resection would have been sufficient to cause permanent cure. Non-vascularized autogenous bone grafts are biologically active grafts with relatively low donor site complications that will be replaced completely by living bone and that are capable of remodeling to fulfill the function need [6, 12–15]. Its cortical composition provides immediate stability to the surrounding joints [16]. The long-term possible complications of this procedure include fracture of the graft, non-union, failure of incorporation of the graft, and stiffness or degenerative arthrosis in the joints [17, 18]. Non-union was observed in the humerus case and delayed union in the proximal femur case, but both patients were treated successfully by rigid fixation. None of our patients had donor site morbidity, and regeneration of the fibula at the donor site occurred within 3 months in the two children. To ensure long-term graft incorporation, the fibular cortical grafts bridged the bone defect after the resection of diaphyseal bone cyst, which should be securely anchored onto the normal bone with a supplemental form of internal fixation; this will promote union of the cortical graft to the host bone [18]. All our patients were supplemented by fixation early or late to achieve this goal.

The ability of the implanted fibular autograft to induce bone formation depends on the early periosteal connections between the autograft and the ends of the resected bone, and it spreads distally along the graft, resulting in autograft to bone union [19]. Osteogenic activity of the fibular autograft is confined to callus formation and trabecular formation within it [19]. We attributed the success of incorporation of the strut fibular graft in our cases to the rich blood supply of the recipient sites and the surrounding well-vascularized soft tissues of the ABC in the proximal femur, humerus and metatarsal.

This will enhance vascularization of the non-vascularized fibular bone graft.

We conclude that strut ipsilateral fibular autograft can be successfully used in the reconstruction of defects observed after resection of giant ABC with excellent clinical and radiological results at final follow-up.