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. 1987 Aug;84(15):5374–5378. doi: 10.1073/pnas.84.15.5374

A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize

R E Dewey *, D H Timothy *, C S Levings III †
PMCID: PMC298858  PMID: 16593863

Abstract

Mitochondrial DNA in the T male-sterile cytoplasm (cms-T) of maize contains an open reading frame (ORF 13) associated with the T type of sterility. Antibodies raised to a chemically synthesized oligopeptide corresponding to ORF 13 were used to establish the expression of a 13-kDa protein from this reading frame. The 13-kDa polypeptide is synthesized uniquely in cms-T maize and purifies with the membrane fraction of T mitochondria. We assign the symbol urf13-T to designate this mitochondrial gene. Presence of the nuclear restorer gene Rf1 in cms-T plants results in a decrease in abundance of 13-kDa protein and alteration in the transcripts of urf13-T.

Keywords: synthetic oligopeptide, immunoprecipitation, protein blotting, nuclear restorer genes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott A. G., Fauron C. M. Structural alterations in a transcribed region of the T type cytoplasmic male sterile maize mitochondrial genome. Curr Genet. 1986;10(10):777–783. doi: 10.1007/BF00405101. [DOI] [PubMed] [Google Scholar]
  2. Cunningham R. S., Gray M. W. Isolation and characterization of 32P-labeled mitochondrial and cytosol ribosomal RNA from germinating wheat embryos. Biochim Biophys Acta. 1977 Apr 4;475(3):476–491. doi: 10.1016/0005-2787(77)90063-6. [DOI] [PubMed] [Google Scholar]
  3. Dewey R. E., Levings C. S., 3rd, Timothy D. H. Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. 1986 Feb 14;44(3):439–449. doi: 10.1016/0092-8674(86)90465-4. [DOI] [PubMed] [Google Scholar]
  4. Forde B. G., Leaver C. J. Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc Natl Acad Sci U S A. 1980 Jan;77(1):418–422. doi: 10.1073/pnas.77.1.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forde B. G., Oliver R. J., Leaver C. J. Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3841–3845. doi: 10.1073/pnas.75.8.3841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gengenbach B. G., Green C. E., Donovan C. M. Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5113–5117. doi: 10.1073/pnas.74.11.5113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldberg D. A. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794–5798. doi: 10.1073/pnas.77.10.5794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982 Jan 1;119(1):142–147. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
  9. Holden M. J., Sze H. Helminthosporium maydis T Toxin Increased Membrane Permeability to Ca in Susceptible Corn Mitochondria. Plant Physiol. 1984 May;75(1):235–237. doi: 10.1104/pp.75.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kheyr-Pour A., Gracen V. E., Everett H. L. Genetics of fertility restoration in the C-group of cytoplasmic male sterility in maize. Genetics. 1981 Jun;98(2):379–388. doi: 10.1093/genetics/98.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  16. Miller R. J., Koeppe D. E. Southern corn leaf blight: susceptible and resistant mitochondria. Science. 1971 Jul 2;173(3991):67–69. doi: 10.1126/science.173.3991.67. [DOI] [PubMed] [Google Scholar]
  17. Payne G., Kono Y., Daly J. M. A Comparison of Purified Host Specific Toxin from Helminthosporium maydis, Race T, and Its Acetate Derivative on Oxidation by Mitochondria from Susceptible and Resistant Plants. Plant Physiol. 1980 May;65(5):785–791. doi: 10.1104/pp.65.5.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Renart J., Sandoval I. V. Western blots. Methods Enzymol. 1984;104:455–460. doi: 10.1016/s0076-6879(84)04114-8. [DOI] [PubMed] [Google Scholar]
  19. Rothbard J. B., Fernandez R., Schoolnik G. K. Strain-specific and common epitopes of gonococcal pili. J Exp Med. 1984 Jul 1;160(1):208–221. doi: 10.1084/jem.160.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Whitaker J. R., Granum P. E. An absolute method for protein determination based on difference in absorbance at 235 and 280 nm. Anal Biochem. 1980 Nov 15;109(1):156–159. doi: 10.1016/0003-2697(80)90024-x. [DOI] [PubMed] [Google Scholar]

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