Correction
After galley proof of the manuscript, we found three mistakes of the nucleotide positions (G222C, G364A and C520T) and codon numbers (Gly74Arg, Gly122Ser and Thr174Ile) that have to be corrected but it was unable to make any change because the publication of this work is on going [1]. After revision, Table two (Table 1 in this manuscript) and some information in the discussion part were changed. There were only 5 novel mutation types found in this study, consisting of 2 nucleotide substitutions (Leu27Pro and Thr174Ile), 2 nucleotide insertions (G insertion between nucleotide 411 and 412 and GG insertion between nucleotide 520 and 521), and 1 nonsense mutation at Glu127.
Table 1.
Results of pncA gene sequencing of 150 M. tuberculosis clinical isolates.
| pncA mutation | ||||
|---|---|---|---|---|
| M. tuberculosis strains (no. of isolates) | MGIT 960 | PZase assay | Nucleotide change | Amino acid change |
| Susceptible (46) | S | + | wild-type | no |
| Susceptible (1) | S | + | T92G | Ile31Ser |
| Susceptible (2) | R | + | wild-type | wild-type |
| Susceptible (1) | R | + | T92C | Ile31Thr |
| MDR-TB (42) | S | + | wild-type | wild-type |
| MDR-TB (9) | S | + | T92C | Ile31Thr |
| MDR-TB (34) | R | - | A(-11)G (1) | no |
| A(-11)C (1) | no | |||
| T56G (1) | Leu19Arg | |||
| T80C (1) | Leu27Pro | |||
| T92G (2) | Ile31Ser | |||
| T104C (1) | Leu35Pro | |||
| T134C (1) | Val45Ala | |||
| G136T (1) | Ala46Ser | |||
| T199C (1) | Ser67Pro | |||
| C211G (8) | His71Asp | |||
| G215A (1) | Cys72Tyr | |||
| G289A (3) | Gly97Ser | |||
| C312G (2) | Ser104Arg | |||
| G322C (1) | Gly108Arg | |||
| G373T (1) | Val125Phe | |||
| G379T (1) | Glu 127 Stop | |||
| G394A (1) | Gly132Ser | |||
| G insertion b/w 411-412 (1) | ||||
| T416G (1) | Val 139 Gly | |||
| C425T (1) | Thr 142 Met | |||
| G436A (1) | Ala 146 Thr | |||
| GG insertion b/w 520-521 (1) | ||||
| C530T (1) | Thr 177 Ile | |||
| MDR-TB (11) | R | + | wild-type | no |
| MDR-TB (4) | R | + | T92C (3) | Ile31Thr |
| T92G (1) | Ile31Ser | |||
We regret any inconvenience that the mistake might have caused. We wish to thank Dr. Claudio Köser, Department of Genetics, University of Cambridge, for bringing this matter to our attention.
Contributor Information
Jirarut Jonmalung, Email: j_enjoy_c@hotmail.com.
Therdsak Prammananan, Email: therdsak@biotec.or.th.
Manoon Leechawengwongs, Email: manoonlee@yahoo.com.
Angkana Chaiprasert, Email: siacp@mahidol.ac.th.
References
- Jonmalung J, Prammananan T, Leechawengwongs M, Chaiprasert A. Surveillance of pyrazinamide susceptibility among multidrug-resistant Mycobacterium tuberculosis isolates from Siriraj Hospital, Thailand. BMC Microbiology. 2010;10:223. doi: 10.1186/1471-2180-10-223. [DOI] [PMC free article] [PubMed] [Google Scholar]