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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Genomics. 2010 Sep 19;96(6):342–351. doi: 10.1016/j.ygeno.2010.09.001

Figure 4. Analyzing the RGMc gene for potential transcriptional enhancers.

Figure 4

A. Map of mouse RGMc gene showing regions that were fused downstream of firefly luciferase (Luc) and the RGMc promoter (coordinates −620 to +118) to test for enhancer activity in differentiating Ad-MyoD-10T½ cells. B. Graphs depict results of luciferase assays after incubation in DM for 0 (white bars) or 24 hr (gray bars) (mean ± S.E. of 3 experiments, each performed in duplicate; values for the RGMc promoter at 24 hr were set to 100 (* - p < 0.01, ** - p < 0.001, vs. t 0)).