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. 1987 Aug;84(16):5570–5574. doi: 10.1073/pnas.84.16.5570

Multiple repressor binding sites in the genome of bacteriophage P1.

M Velleman, B Dreiseikelmann, H Schuster
PMCID: PMC298904  PMID: 3039493

Abstract

After digestion of bacteriophage P1 DNA with EcoRI in the presence of P1 repressor, 6 repressor binding sites were identified in 5 of 26 EcoRI fragments. Binding sites were localized by the decreased mobility of DNA fragment-repressor complexes during electrophoresis and by DNase protection ("footprinting") analysis. The repressor binding sites, or operators, comprise a 17-base-pair-long consensus sequence lacking symmetrical elements. Three operators can be related to known genes, whereas the function of the others is still unknown. The mutant P1 bac, rendering ban expression constitutive, is identified as an operator-constitutive mutation of the ban operon.

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