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. 1987 Aug;84(16):5750–5753. doi: 10.1073/pnas.84.16.5750

Construction of a Leydig cell line synthesizing testosterone under gonadotropin stimulation: a complex endocrine function immortalized by cell hybridization.

C Finaz, A Lefèvre, D Dampfhoffer
PMCID: PMC298940  PMID: 3039504

Abstract

Hybridization between a mouse Leydig tumor cell line, MA-10, which produces cyclic AMP and progesterone under human chorionic gonadotropin (hCG) stimulation, and freshly isolated mouse Leydig cells gave rise to 54 hybrid clones, one of which, LK17, was capable of hCG-stimulated testosterone production. Subcloning of this hybrid resulted in the emergence of a subclone, K9, whose testosterone production is more than 10 times that of parent clone LK17, after hCG stimulation, with an ED50 of 37 pM. Testosterone synthesis by K9 cells was multiplied by 25 after gonadotropin stimulation, and binding of hCG declined after prolonged exposure to the hormone. These similarities with murine Leydig cells in primary culture make the K9 clone an attractive alternative for physiological studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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