Abstract
A comparison is presented of the structures obtained around the iron atom in deoxyhemoglobin (Hb). The data come from extended x-ray absorption fine structure (EXAFS) studies of the iron, which gave Fe-porphyrin nitrogen distances of 2.06 +/- 0.01 A, and from the most recent high-resolution x-ray crystallographic study, which gave exactly the same distance--2.06 +/- 0.02 A. The distance of Fe above the plane of the porphyrin nitrogens was 0.38 +/- 0.04 A from the crystallographic study; this value is not far from the upper limit of the distances 0.20 +/- (0.10)0.20 A calculated from the EXAFS experiment by triangulation. These distances above the nitrogen plane are shorter than those estimated in the earliest x-ray structures.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bolton W., Perutz M. F. Three dimensional fourier synthesis of horse deoxyhaemoglobin at 2.8 Angstrom units resolution. Nature. 1970 Nov 7;228(5271):551–552. doi: 10.1038/228551a0. [DOI] [PubMed] [Google Scholar]
- Eisenberger P., Shulman R. G., Kincaid B. M., Brown G. S., Ogawa S. Extended X-ray absorption fine structure determination of iron nitrogen distances in haemoglobin. Nature. 1978 Jul 6;274(5666):30–34. doi: 10.1038/274030a0. [DOI] [PubMed] [Google Scholar]
- Fermi G., Perutz M. F., Shaanan B., Fourme R. The crystal structure of human deoxyhaemoglobin at 1.74 A resolution. J Mol Biol. 1984 May 15;175(2):159–174. doi: 10.1016/0022-2836(84)90472-8. [DOI] [PubMed] [Google Scholar]
- Fermi G. Three-dimensional fourier synthesis of human deoxyhaemoglobin at 2-5 A resolution: refinement of the atomic model. J Mol Biol. 1975 Sep 15;97(2):237–256. doi: 10.1016/s0022-2836(75)80037-4. [DOI] [PubMed] [Google Scholar]
- Perutz M. F., Hasnain S. S., Duke P. J., Sessler J. L., Hahn J. E. Stereochemistry of iron in deoxyhaemoglobin. Nature. 1982 Feb 11;295(5849):535–538. doi: 10.1038/295535a0. [DOI] [PubMed] [Google Scholar]
- Perutz M. F. Stereochemistry of cooperative effects in haemoglobin. Nature. 1970 Nov 21;228(5273):726–739. doi: 10.1038/228726a0. [DOI] [PubMed] [Google Scholar]
- Shulman R. G. On extended x-ray absorption fine structure studies of hemoglobin. Proc Natl Acad Sci U S A. 1987 Feb;84(4):973–974. doi: 10.1073/pnas.84.4.973. [DOI] [PMC free article] [PubMed] [Google Scholar]