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. Author manuscript; available in PMC: 2011 Nov 23.
Published in final edited form as: Vaccine. 2010 Oct 8;28(50):8015–8025. doi: 10.1016/j.vaccine.2010.08.106

Fig 7. NS-LAIV candidates are attenuated in ferrets and protect from challenge.

Fig 7

Ferrets were inoculated intranasally with 106.5 TCID50 of the various recombinant viruses. (A) Virus shedding from the nasal airway of the infected ferrets was determined by TCID50 assay of nasal washes collected at 1, 3, 5 and 7 dpi. (B) Body weight of the inoculated ferrets (n=3-4/group) was recorded on alternate days and is represented as the mean percent of initial body weight (day 0). (C) Neutralizing antibody levels in sera isolated 6.5 weeks post immunization was determined by micro-neutralization assay. (D) 6.5 weeks post immunization, ferrets were challenged with 106 PFU of A/Mexico/4482/2009 (H1N1pdm). Virus shedding from the nasal airway was determined by plaque assay of nasal washes collected at 1, 3, 5 and 7 dpc. The average of each group is shown and the error bars represent SEM. If virus titers were below the LOD (1.5 log10) they are shown as 1 log10, however they lack error bars (A, & D).