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. 1987 Oct;84(19):6810–6814. doi: 10.1073/pnas.84.19.6810

Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing.

B Séraphin 1, A Boulet 1, M Simon 1, G Faye 1
PMCID: PMC299174  PMID: 3309947

Abstract

We have constructed a respiring yeast strain devoid of mitochondrial introns to screen nuclear pet- mutants for those that play a direct role in mitochondrial intron excision. Intron-less mitochondria are introduced by cytoduction into pet- strains that have been made rho0; cytoductants therefrom recover respiratory competency if the original pet- mutation is required only for mitochondrial splicing. By this means, we have identified 11 complementation groups of such genes. Their total number may be estimated as about 18.

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Selected References

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