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. 1987 Oct;84(19):6825–6829. doi: 10.1073/pnas.84.19.6825

Heterologous enzyme function in Escherichia coli and the selection of genes encoding the catalytic RNA subunit of RNase P.

N P Lawrence 1, A Richman 1, R Amini 1, S Altman 1
PMCID: PMC299177  PMID: 2443911

Abstract

The gene for the catalytic RNA subunit of RNase P has been isolated from several Enterobacteriaceae by complementation of an Escherichia coli strain that is temperature-sensitive for RNase P activity. The selection procedure relies on the ability of the heterologous gene products to function enzymatically in E. coli. This procedure obviates the need for positive results in DNA blot hybridization experiments or for the purification of holoenzyme to identify the RNA component of RNase P and its corresponding gene from organisms other than E. coli. Comparisons of the variations in sequences provide the basis for a refined two-dimensional model of the secondary structure of M1 RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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