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. 1987 Oct;84(19):6944–6947. doi: 10.1073/pnas.84.19.6944

Direct neurotrophic action of glycyl-L-glutamine in the maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat.

G B Koelle 1, U J Sanville 1, N S Thampi 1
PMCID: PMC299201  PMID: 3477818

Abstract

Intracarotid infusion of glycyl-L-glutamine (Gly-Gln) was shown previously to oppose the fall in the acetylcholinesterase and butyrylcholinesterase contents of the cat superior cervical ganglion (SCG) that otherwise follows preganglionic denervation. However, its effect was demonstrable only on the vascularly remote left SCG but not on the directly infused right SCG. Accordingly, it was concluded that a metabolite of Gly-Gln, formed in the blood, is an active neurotrophic factor. Glycyl-L-glutamic acid and L-glutamic acid were subsequently found to have a similar but less marked effect on both SCG. In the present study an alternative explanation has been tested: that Gly-Gln must combine slowly with some component of plasma to enable it to penetrate the ganglion cells and exert its neurotrophic effect. Findings are consistent with the latter proposal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cupello A., Hydén H. gamma-Aminobutyric acid (GABA) removal from the synaptic cleft: a postsynaptic event? Cell Mol Neurobiol. 1986 Mar;6(1):1–16. doi: 10.1007/BF00742972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis R., Koelle G. B. Electron microscope localization of acetylcholinesterase and butyrylcholinesterase in the superior cervical ganglion of the cat. II. Preganglionically denervated ganglion. J Cell Biol. 1981 Mar;88(3):581–590. doi: 10.1083/jcb.88.3.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELLMAN G. L., COURTNEY K. D., ANDRES V., Jr, FEATHER-STONE R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 Jul;7:88–95. doi: 10.1016/0006-2952(61)90145-9. [DOI] [PubMed] [Google Scholar]
  4. Koelle G. B., Davis R., Koelle W. A. Effects of aldehyde fixation and of preganglionic denervation on acetylcholinesterase and butyrocholinesterase of cat autonomic ganglia. J Histochem Cytochem. 1974 Apr;22(4):244–251. doi: 10.1177/22.4.244. [DOI] [PubMed] [Google Scholar]
  5. Koelle G. B., Ruch G. A. Demonstration of a neurotrophic factor for the maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat. Proc Natl Acad Sci U S A. 1983 May;80(10):3106–3110. doi: 10.1073/pnas.80.10.3106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Koelle G. B., Ruch G. A., Rickard K. K., Sanville U. J. Regeneration of cholinesterases in the stellate and normal and denervated superior cervical ganglion of the cat following inactivation by sarin. J Neurochem. 1982 Jun;38(6):1695–1698. doi: 10.1111/j.1471-4159.1982.tb06651.x. [DOI] [PubMed] [Google Scholar]
  7. Koelle G. B., Sanville U. J., Rickard K. K., Williams J. E. Partial characterization of the neurotrophic factor for maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat in vivo. Proc Natl Acad Sci U S A. 1984 Oct;81(20):6539–6542. doi: 10.1073/pnas.81.20.6539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koelle G. B., Sanville U. J., Thampi N. S., Wall S. J. L-glutamic acid, a neurotrophic factor for maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2751–2754. doi: 10.1073/pnas.83.8.2751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koelle G. B., Sanville U. J., Wall S. J. Glycyl-L-glutamine, a precursor, and glycyl-L-glutamic acid, a neurotrophic factor for maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat in vivo. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5213–5217. doi: 10.1073/pnas.82.15.5213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. McDonald J. K., Zeitman B. B., Ellis S. Detection of a lysosomal carboxypeptidase and a lysosomal dipeptidase in highly-purified dipeptidyl aminopeptidase I (cathepsin C) and the elimination of their activities from preparations used to sequence peptides. Biochem Biophys Res Commun. 1972 Jan 14;46(1):62–70. doi: 10.1016/0006-291x(72)90630-4. [DOI] [PubMed] [Google Scholar]
  12. Nagata Y., Yokoi Y., Tsukada Y. Studies on free amino acid metabolism in excised cervical sympathetic ganglia from the rat. J Neurochem. 1966 Dec;13(12):1421–1431. doi: 10.1111/j.1471-4159.1966.tb04303.x. [DOI] [PubMed] [Google Scholar]
  13. Parish D. C., Smyth D. G., Normanton J. R., Wolstencroft J. H. Glycyl glutamine, an inhibitory neuropeptide derived from beta-endorphin. Nature. 1983 Nov 17;306(5940):267–270. doi: 10.1038/306267a0. [DOI] [PubMed] [Google Scholar]
  14. Trapeznikova S. S., Paskhina T. S. Vydelenie, ochistka i svoistva karboksipeptidazy (kininazy) iz syvorotki krovi cheloveka i krolika. Biokhimiia. 1968 Sep-Oct;33(5):1012–1022. [PubMed] [Google Scholar]

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