TABLE 1 .
H1N1 reassortant genotypes recovered after 10 sequential passages from differentiated NHBE cultures coinfected with seasonal NJ/15 and pandemic TN/560 (H1N1) influenza viruses
| Coinfection group | Genotypea |
No. of isolates with the described genotype/total no. of viruses examined | Abbreviation | Molecular change(s) during coinfectionb | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| PB2 | PB1 | PA | HA | NP | NA | M | NS | ||||
| 10% NJ/15-90% TN/560 | TN | TN | TN | TN | TN | TN | TN | TN | 30/30 | G1 | PA L295P; HA K154Q |
| 50% NJ/15-50% TN/560 | TN | TN | TN | TN | TN | TN | TN | TN | 31/31 | PA L295P | |
| 90% NJ/15-10% TN/560 | TN | TN | TN | NJ | TN | TN | TN | TN | 24/26c | G2 | PB2 D87N |
a
NJ, gene derived from the NJ/15 parental virus; TN, gene derived from the TN/560 parental virus.
b
Molecular changes are shown based on the sequences of the ~20 to 25% of plaque-purified viruses isolated from NHBE culture supernatants from three coinfection groups (H1 HA numbering) (20).
c
2 H1N1 clones had a mixed NJ/TN genotype for the HA gene.