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. 1987 Nov;84(21):7600–7604. doi: 10.1073/pnas.84.21.7600

Molecular analysis of X chromosome dosage compensation in Caenorhabditis elegans.

L M Donahue 1, B A Quarantillo 1, W B Wood 1
PMCID: PMC299347  PMID: 3478715

Abstract

We used a convenient quantitative dot blot assay to measure transcript levels for two X chromosome-linked genes, myo-2 and act-4, in the nematode Caenorhabditis elegans. We show that there is dosage compensation of transcript levels for these two genes between XX hermaphrodites and X0 males and that a mutation in the dpy-21 gene, postulated from genetic analysis to be involved in control of X chromosome expression, can affect these transcript levels in the manner predicted. However, we observe the dpy-21 effects only at some stages of the life cycle and not at others. These results are generally consistent with earlier genetic and molecular evidence.

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Selected References

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