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. 1987 Nov;84(22):7807–7811. doi: 10.1073/pnas.84.22.7807

Identification of a transposon Tn7-dependent DNA-binding activity that recognizes the ends of Tn7.

R L McKown 1, C S Waddell 1, L K Arciszewska 1, N L Craig 1
PMCID: PMC299398  PMID: 2825163

Abstract

The bacterial transposon Tn7 is distinguished by its capacity for high-frequency transposition to a specific site in the Escherichia coli chromosome. tnsB is one of the five Tn7-encoded transposition genes. We have identified in vitro a tnsB-dependent DNA binding activity that interacts specifically with cis-acting transposition sequences at the Tn7 termini. Although the left and right termini of Tn7 are structurally distinct, each end contains several copies of a closely homologous 22-base-pair sequence. We present results indicating that this 22-base-pair repeat sequence is recognized by the tnsB-dependent binding activity.

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