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A method to probe interactions between modified CP’s and KS’s of type II FAS and PKS pathways. A) A comparison of type II biosyntheses between FAS in E. coli and enterocin biosyntheis in S. maritimus. B) Apo-ACP is incubated with CoaA, CoaD, CoaE, and Sfp in the presence of ATP and pantetheine analog 1-9 to generate crypto-ACP. Crypto-ACP reacts with the active site cysteine of the ketosynthase to form ACP-KAS fusion product.
