Abstract
The effect of horseradish peroxidase/H2O2 in organic medium (dioxane/aqueous buffer, pH 5, 95:5) on the depolymerization of synthetic (dehydrogenatively polymerized) lignin was reinvestigated. In contrast to previous claims [Dordick, J. S., Marletta, M. A. & Klibanov, A. M. (1986) Proc. Natl. Acad. Sci. USA 83, 6255-6257], our results demonstrated that vigorous depolymerization of this substrate did not occur. Further, during this treatment ferulic acid was not a significant biodegradation product.
Keywords: dehydrogenatively polymerized lignin, enzymes in nonaqueous solvents, ligninase
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dordick J. S., Marletta M. A., Klibanov A. M. Peroxidases depolymerize lignin in organic media but not in water. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6255–6257. doi: 10.1073/pnas.83.17.6255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kirk T. K., Connors W. J., Bleam R. D., Hackett W. F., Zeikus J. G. Preparation and microbial decomposition of synthetic [14C]ligins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2515–2519. doi: 10.1073/pnas.72.7.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tien M., Kirk T. K. Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds. Science. 1983 Aug 12;221(4611):661–663. doi: 10.1126/science.221.4611.661. [DOI] [PubMed] [Google Scholar]