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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Hepatology. 2010 Apr;51(4):1391–1400. doi: 10.1002/hep.23431

Fig. 5. BrdU incorporation indicates that modulations in Notch signaling do not affect the proliferation index of cholangiocytes.

Fig. 5

(A–J) Representative sections from P30 mice labeled for 4 days with BrdU administered via drinking water. Paraffin sections were stained for wsCK to mark the cholangiocytes, BrdU to mark DNA replication and Hoechst to mark DNA. wsCK+ cells and double wsCK/BrdU+ cells were counted from regions in the hilum (A–E) and periphery (F–J). (K–L) Ratio of double positive to total wsCK+ cells, absolute numbers in Supplementary Table 3. (K) Peripheral analysis of P15 mice with 1-hour BrdU pulse. (L) Peripheral analysis of P26 mice administered BrdU for 4 days and sacrificed at P30. NICD mice have a significantly decreased percent proliferation as compared to control [Alb-Cre]. Arrowheads, representative dual-labeled cell. Percent proliferation was calculated per region analyzed and averaged. Statistical analysis was performed using a one-way ANOVA with Dunnett’s post-test. Error bars are standard error of the mean between individual regions. *P<0.05. Scale bar = 100 µm.