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. 1987 Dec;84(24):8763–8767. doi: 10.1073/pnas.84.24.8763

Comparative mutational analysis of wild-type and stretched tRNA3(Leu) gene promoters.

P Fabrizio 1, A Coppo 1, P Fruscoloni 1, P Benedetti 1, G Di Segni 1, G P Tocchini-Valentini 1
PMCID: PMC299630  PMID: 3321052

Abstract

We demonstrate that, when the yeast tRNA(3Leu) gene is stretched so that the distance between the two portions of the intragenic promoter is increased to 365 base pairs, the A and B blocks remain functional. Mutations in the A block, which show a weak phenotype when inserted in the wild type, exert a dramatic effect when inserted into the stretched gene. Experiments with extensively purified transcription factor tau indicate that the tau B-B block interaction is not influenced by A-B distance; only the ability of tau A to interact with A block sequences is affected, possibly because of the additional free-energy cost of forming a large loop of the intervening DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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