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. 1987 Dec;84(24):8839–8843. doi: 10.1073/pnas.84.24.8839

Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.

N F Lue 1, R D Kornberg 1
PMCID: PMC299646  PMID: 3321057

Abstract

A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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