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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nature. 2010 Sep 29;468(7324):664–668. doi: 10.1038/nature09479

Figure 1. U1 AMO binds to the 5′ sequence of U1 snRNA and inhibits its splicing activity.

Figure 1

(a) HeLa cells were transfected with the indicated concentrations of control and U1 AMO for 8 hrs. RNase H protection assay was performed using total cell extracts and U1 snRNA was detected by Northern blotting.

(b) In situ hybridization was performed on HeLa cells transfected with varying concentrations of U1 AMO as indicated for 8 hrs using a biotin-labeled LNA probe to the U1 snRNA (left panels) followed by fluorescent Alexa Fluor 594 streptavidin conjugate detection. Nuclei were visualized with DAPI (middle panels) and merged images are shown (right panels).

(c) [α–32P] UTP-labeled Ad2ΔIVS pre-mRNA was spliced in vitro in the presence of control or U1 AMOs at the indicated concentrations. Splicing product identities are depicted to the right of the gel.