Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1987 Dec;84(24):9099–9102. doi: 10.1073/pnas.84.24.9099

Genetic analysis of tumorigenesis: a conserved region in the human and Chinese hamster genomes contains genetically identified tumor-suppressor genes.

G Stenman 1, R Sager 1
PMCID: PMC299699  PMID: 3321067

Abstract

Regional chromosome homologies were found in a comparison of human 11p with Chinese hamster 3p. By use of probes that recognize six genes of human 11p (INS, CAT, HBBC, CALC, PTH, and HRAS), the corresponding genes were localized by in situ hybridization on Chinese hamster chromosome 3. INS and CAT were located close to the centromere on 3p, whereas HBBC, CALC, and PTH were at 3q3-4 and HRAS at 3q4. Extensive prior data from chromosome studies of tumorigenic and tumor-derived Chinese hamster cells have suggested the presence of a tumor-suppressor gene on 3p. Two tumor-suppressor genes have been described on human 11p, one linked to CAT and one to INS. The present study raises the possibility that the Chinese hamster suppressor may be closely linked to INS or CAT.

Full text

PDF
9099

Images in this article

9101Image
on p.9101
9101Image
on p.9101
9101Image
on p.9101
9101Image
on p.9101
9101Image
on p.9101
9101Image
on p.9101
9101Image
on p.9101
9101Image
on p.9101

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adair G. M., Stallings R. L., Siciliano M. J. Chromosomal rearrangements and gene expression in CHO cells: mapping of alleles for eight enzyme loci on CHO chromosomes Z3, Z4, Z5, and Z7. Somat Cell Mol Genet. 1984 May;10(3):283–295. doi: 10.1007/BF01535250. [DOI] [PubMed] [Google Scholar]
  2. Anisowicz A., Bardwell L., Sager R. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7188–7192. doi: 10.1073/pnas.84.20.7188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chandler M. E., Yunis J. J. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes. Cytogenet Cell Genet. 1978;22(1-6):352–356. doi: 10.1159/000130970. [DOI] [PubMed] [Google Scholar]
  4. Craig R. W., Sager R. Suppression of tumorigenicity in hybrids of normal and oncogene-transformed CHEF cells. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2062–2066. doi: 10.1073/pnas.82.7.2062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellis R. W., DeFeo D., Maryak J. M., Young H. A., Shih T. Y., Chang E. H., Lowy D. R., Scolnick E. M. Dual evolutionary origin for the rat genetic sequences of Harvey murine sarcoma virus. J Virol. 1980 Nov;36(2):408–420. doi: 10.1128/jvi.36.2.408-420.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Franchini G., Even J., Sherr C. J., Wong-Staal F. onc sequences (v-fes) of Snyder-Theilen feline sarcoma virus are derived from noncontiguous regions of a cat cellular gene (c-fes). Nature. 1981 Mar 12;290(5802):154–157. doi: 10.1038/290154a0. [DOI] [PubMed] [Google Scholar]
  7. Gadi I. K., Harrison J. J., Sager R. Genetic analysis of tumorigenesis: XVI. Chromosome changes in azacytidine- and insulin-induced tumorigenesis. Somat Cell Mol Genet. 1984 Sep;10(5):521–529. doi: 10.1007/BF01534856. [DOI] [PubMed] [Google Scholar]
  8. Grzeschik K. H., Kazazian H. H. Report of the Committee on the Genetic Constitution of Chromosomes 10, 11, and 12. Cytogenet Cell Genet. 1985;40(1-4):179–205. doi: 10.1159/000132174. [DOI] [PubMed] [Google Scholar]
  9. Harper M. E., Saunders G. F. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. doi: 10.1007/BF00327364. [DOI] [PubMed] [Google Scholar]
  10. Heinrich G., Kronenberg H. M., Potts J. T., Jr, Habener J. F. Gene encoding parathyroid hormone. Nucleotide sequence of the rat gene and deduced amino acid sequence of rat preproparathyroid hormone. J Biol Chem. 1984 Mar 10;259(5):3320–3329. [PubMed] [Google Scholar]
  11. Jacobs J. W., Goodman R. H., Chin W. W., Dee P. C., Habener J. F., Bell N. H., Potts J. T., Jr Calcitonin messenger RNA encodes multiple polypeptides in a single precursor. Science. 1981 Jul 24;213(4506):457–459. doi: 10.1126/science.6264603. [DOI] [PubMed] [Google Scholar]
  12. Kitchin R. M., Gadi I. K., Smith B. L., Sager R. Genetic analysis of tumorigenesis: X. Chromosome studies of transformed mutants and tumor-derived CHEF/18 cells. Somatic Cell Genet. 1982 Sep;8(5):677–689. doi: 10.1007/BF01542860. [DOI] [PubMed] [Google Scholar]
  13. Kitchin R. M., Sager R. Genetic analysis of tumorigenesis: VI. Chromosome rearrangements in tumors derived from diploid premalignant Chinese hamster cells in nude mice. Somatic Cell Genet. 1980 Sep;6(5):615–630. doi: 10.1007/BF01538641. [DOI] [PubMed] [Google Scholar]
  14. Kozak C. A., Sears J. F., Hoggan M. D. Genetic mapping of the mouse oncogenes c-Ha-ras-1 and c-fes to chromosome 7. J Virol. 1983 Jul;47(1):217–220. doi: 10.1128/jvi.47.1.217-220.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lau C. C., Gadi I. K., Kalvonjian S., Anisowicz A., Sager R. Plasmid-induced "hit-and-run" tumorigenesis in Chinese hamster embryo fibroblast (CHEF) cells. Proc Natl Acad Sci U S A. 1985 May;82(9):2839–2843. doi: 10.1073/pnas.82.9.2839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lomedico P., Rosenthal N., Efstratidadis A., Gilbert W., Kolodner R., Tizard R. The structure and evolution of the two nonallelic rat preproinsulin genes. Cell. 1979 Oct;18(2):545–558. doi: 10.1016/0092-8674(79)90071-0. [DOI] [PubMed] [Google Scholar]
  17. Ma N. S., Gerhard D. S., Housman D. E., Orkin S., Bruns G. Owl monkey gene mapping: the assignment of gene loci for catalase, beta-globin gene cluster, HRAS1, insulin, and parathyroid hormone. Cytogenet Cell Genet. 1986;43(1-2):57–68. doi: 10.1159/000132298. [DOI] [PubMed] [Google Scholar]
  18. Meyers D. A., Beaty T. H., Maestri N. E., Antonarakis S. E., Kazazian H. H., Jr Multipoint mapping studies of the beta-globin, insulin, and c-Ha-ras-1 loci on 11p. Am J Hum Genet. 1986 Oct;39(4):539–541. [PMC free article] [PubMed] [Google Scholar]
  19. Nadeau J. H., Taylor B. A. Lengths of chromosomal segments conserved since divergence of man and mouse. Proc Natl Acad Sci U S A. 1984 Feb;81(3):814–818. doi: 10.1073/pnas.81.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ray F. A., Bartholdi M. F., Kraemer P. M., Cram L. S. Spontaneous in vitro neoplastic evolution: recurrent chromosome changes of newly immortalized Chinese hamster cells. Cancer Genet Cytogenet. 1986 Mar 1;21(1):35–51. doi: 10.1016/0165-4608(86)90199-8. [DOI] [PubMed] [Google Scholar]
  21. Rougeon F., Mach B. Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro. Gene. 1977 May;1(3-4):229–239. doi: 10.1016/0378-1119(77)90047-6. [DOI] [PubMed] [Google Scholar]
  22. Sager R., Kovac P. E. Genetic analysis of tumorigenesis: I. Expression of tumor-forming ability in hamster hybrid cell lines. Somatic Cell Genet. 1978 May;4(3):375–392. doi: 10.1007/BF01542849. [DOI] [PubMed] [Google Scholar]
  23. Scrable H. J., Witte D. P., Lampkin B. C., Cavenee W. K. Chromosomal localization of the human rhabdomyosarcoma locus by mitotic recombination mapping. Nature. 1987 Oct 15;329(6140):645–647. doi: 10.1038/329645a0. [DOI] [PubMed] [Google Scholar]
  24. Stallings R. L., Crawford B. D., Black R. J., Chang E. H. Assignment of RAS proto-oncogenes in Chinese hamsters: implications for mammalian gene linkage conservation and neoplasia. Cytogenet Cell Genet. 1986;43(1-2):2–5. doi: 10.1159/000132289. [DOI] [PubMed] [Google Scholar]
  25. Stallings R. L., Munk A. C., Longmire J. L., Jett J. H., Wilder M. E., Siciliano M. J., Adair G. M., Crawford B. D. Oncogenes and linkage groups: conservation during mammalian chromosome evolution. Chromosoma. 1985;92(2):156–163. doi: 10.1007/BF00328468. [DOI] [PubMed] [Google Scholar]
  26. Stenman G., Delorme E. O., Lau C. C., Sager R. Transfection with plasmid pSV2gptEJ induces chromosome rearrangements in CHEF cells. Proc Natl Acad Sci U S A. 1987 Jan;84(1):184–188. doi: 10.1073/pnas.84.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Weissman B. E., Saxon P. J., Pasquale S. R., Jones G. R., Geiser A. G., Stanbridge E. J. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression. Science. 1987 Apr 10;236(4798):175–180. doi: 10.1126/science.3031816. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES