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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Differentiation. 2011 Jan;81(1):68–77. doi: 10.1016/j.diff.2010.09.181

Figure 5.

Figure 5

Structure of the PrPC mRNA and pSUPER.neo vector used to target PrPC mRNA expression. (A) Bovine PrPC mRNA showing location of siRNA target sequence, probe and primer location for QPCR. pSUPER.neo vectordriven by the H1 RNA promoter, yields mRNA that contains two 19-base pallindromic targeting sequences separated by nine bases that form a hairpin loop after folding of the pallindromes to form a double-stranded RNA. (B) Control vector was identical, save for substitution of a scrambled version of the targeting sequence.