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. 1987 Dec;84(24):9204–9208. doi: 10.1073/pnas.84.24.9204

Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2.

J A Lumadue 1, A B Glick 1, F H Ruddle 1
PMCID: PMC299721  PMID: 3480538

Abstract

Among the earliest expressed antigens on the surface of activated human lymphocytes is the surface antigen 4F2. We have used DNA-mediated gene transfer and fluorescence-activated cell sorting to obtain cell lines that contain the gene encoding the large subunit of the human 4F2 antigen in a mouse L-cell background. Human DNAs cloned from these cell lines were subsequently used as hybridization probes to isolate a full-length cDNA clone expressing 4F2. Sequence analysis of the coding region has revealed an amino acid sequence of 529 residues. Hydrophobicity plotting has predicted a probable structure for the protein that includes an external carboxyl terminus, an internal leader sequence, a single hydrophobic transmembrane domain, and two possible membrane-associated domains. The 4F2 cDNA detects a single 1.8-kilobase mRNA in T-cell and B-cell lines. RNA gel blot analysis of RNA derived from quiescent and serum-stimulated Swiss 3T3 fibroblasts reveals a cell-cycle modulation of 4F2 gene expression: the mRNA is present in quiescent fibroblasts but increases 8-fold 24-36 hr after stimulation, at the time of maximal DNA synthesis.

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Selected References

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