Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1987 Dec;84(24):9219–9222. doi: 10.1073/pnas.84.24.9219

Unexpected binding of an octapeptide to the angiotensin II receptor.

R L Soffer 1, S Bandyopadhyay 1, E Rosenberg 1, P Hoeprich 1, A Teitelbaum 1, T Brunck 1, C B Colby 1, C Gloff 1
PMCID: PMC299724  PMID: 3480539

Abstract

An octapeptide, TBI-22 (Lys-Gly-Val-Tyr-Ile-His-Ala-Leu), inhibited binding of angiotensin II by a solubilized angiotensin receptor partially purified from rabbit liver. This inhibition appears to result from competition for binding to the same receptor. Radioiodinated TBI-22, like angiotensin II, bound to the solubilized receptor with an affinity such that the binding was inhibited 50% by unlabeled TBI-22 or angiotensin II at nanomolar concentrations. The binding reaction, like that for angiotensin II, required p-chloromercuriphenylsulfonic acid and was reversed in the presence of dithiothreitol. TBI-22 and angiotensin II share the sequence Val-Tyr-Ile-His; this tetrapeptide alone, however, did not inhibit binding of angiotensin II. Replacement of the tyrosine residue by aspartic acid in TBI-22 greatly reduced the ability of the peptide to compete with angiotensin II for binding, suggesting an important contribution of this residue to the configuration required for recognition by the receptor.

Full text

PDF
9219

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bost K. L., Smith E. M., Blalock J. E. Similarity between the corticotropin (ACTH) receptor and a peptide encoded by an RNA that is complementary to ACTH mRNA. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1372–1375. doi: 10.1073/pnas.82.5.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Capponi A. M., Catt K. J. Angiotensin II receptors in adrenal cortex and uterus. Binding and activation properties of angiotensin analogues. J Biol Chem. 1979 Jun 25;254(12):5120–5127. [PubMed] [Google Scholar]
  3. Cemassieux S., Boucher R., Crisé C., Genest J. Purification and characterization of tonin. Can J Biochem. 1976 Sep;54(9):788–795. doi: 10.1139/o76-113. [DOI] [PubMed] [Google Scholar]
  4. Das M., Soffer R. L. Pulmonary angiotensin-converting enzyme antienzyme antibody. Biochemistry. 1976 Nov 16;15(23):5088–5094. doi: 10.1021/bi00668a022. [DOI] [PubMed] [Google Scholar]
  5. Ferguson R. K., Turini G. A., Brunner H. R., Gavras H., McKinstry D. N. A specific orally active inhibitor of angiotensin-converting enzyme in man. Lancet. 1977 Apr 9;1(8015):775–778. doi: 10.1016/s0140-6736(77)92958-0. [DOI] [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glossmann H., Baukal A. J., Catt K. J. Properties of angiotensin II receptors in the bovine and rat adrenal cortex. J Biol Chem. 1974 Feb 10;249(3):825–834. [PubMed] [Google Scholar]
  8. Gocke D. J., Gerten J., Sherwood L. M., Laragh J. H. Physiological and pathological variations of plasma angiotensin II in man. Correlation with renin activity and sodium balance. Circ Res. 1969 May;24(5 Suppl):131–148. [PubMed] [Google Scholar]
  9. HELMER O. M. Differentiation between two forms of angiotonin by means of spirally cut strips of rabbit aorta. Am J Physiol. 1957 Mar;188(3):571–577. doi: 10.1152/ajplegacy.1957.188.3.571. [DOI] [PubMed] [Google Scholar]
  10. Heinrikson R. L., Meredith S. C. Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate. Anal Biochem. 1984 Jan;136(1):65–74. doi: 10.1016/0003-2697(84)90307-5. [DOI] [PubMed] [Google Scholar]
  11. Hoeprich P. D., Jr, Doolittle R. F. Dimeric half-molecules of human fibrinogen are joined through disulfide bonds in an antiparallel orientation. Biochemistry. 1983 Apr 26;22(9):2049–2055. doi: 10.1021/bi00278a003. [DOI] [PubMed] [Google Scholar]
  12. Kaiser E., Colescott R. L., Bossinger C. D., Cook P. I. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. Anal Biochem. 1970 Apr;34(2):595–598. doi: 10.1016/0003-2697(70)90146-6. [DOI] [PubMed] [Google Scholar]
  13. Lin S. Y., Goodfriend T. L. Angiotensin receptors. Am J Physiol. 1970 May;218(5):1319–1328. doi: 10.1152/ajplegacy.1970.218.5.1319. [DOI] [PubMed] [Google Scholar]
  14. Marshall G. R., Vine W., Needlemann P. A specific competitive inhibitor of angiotensin II. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1624–1630. doi: 10.1073/pnas.67.3.1624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ondetti M. A., Rubin B., Cushman D. W. Design of specific inhibitors of angiotensin-converting enzyme: new class of orally active antihypertensive agents. Science. 1977 Apr 22;196(4288):441–444. doi: 10.1126/science.191908. [DOI] [PubMed] [Google Scholar]
  16. Pals D. T., Masucci F. D., Sipos F., Denning G. S., Jr A specific competitive antagonist of the vascular action of angiotensin. II. Circ Res. 1971 Dec;29(6):664–672. doi: 10.1161/01.res.29.6.664. [DOI] [PubMed] [Google Scholar]
  17. Patchett A. A., Harris E., Tristram E. W., Wyvratt M. J., Wu M. T., Taub D., Peterson E. R., Ikeler T. J., ten Broeke J., Payne L. G. A new class of angiotensin-converting enzyme inhibitors. Nature. 1980 Nov 20;288(5788):280–283. doi: 10.1038/288280a0. [DOI] [PubMed] [Google Scholar]
  18. Phillips M. I. Angiotensin in the brain. Neuroendocrinology. 1978;25(6):354–377. doi: 10.1159/000122756. [DOI] [PubMed] [Google Scholar]
  19. Sen I., Bull H. G., Soffer R. L. Isolation of an angiotensin II-binding protein from liver. Proc Natl Acad Sci U S A. 1984 Mar;81(6):1679–1683. doi: 10.1073/pnas.81.6.1679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sen I., Jim K. F., Soffer R. L. Solubilization and characterization of an angiotensin II binding protein from liver. Eur J Biochem. 1983 Oct 17;136(1):41–49. doi: 10.1111/j.1432-1033.1983.tb07702.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES