Figure 6.

Lucifer Yellow (LY) uptake in the presence and absence of Hep-MION was investigated in transcellular transport experiments, with and without the magnetic field. The vesicles inside the cells became labeled upon incubation with the fluorescent fluid phase marker, LY, visible using the FITC excitation/emission channel of the Nikon TE2000S epifluorescence microscope (100 × objective). (A) Control experiments without Hep-MION. (B) Experimental treatment group with Hep-MION (0.2575 mg/mL). Scale bar of 10 µm and nucleus of each cell as “N” are displayed in each image. For display, contrast was enhanced and the circumference of each cell (in white) was manually outlined with Adobe Photoshop.