Figure 1.—

Loss of a receptor-type guanylyl cyclase GCY-22 suppresses the chemotaxis defect of daf-18 mutants. (A) pe902, pe905, pe917, and pe922 were obtained in a suppressor screen for mutations that rescue the chemotaxis defect of daf-18(e1375) mutants. Naive animals were tested for NaCl chemotaxis. A deletion mutation gcy-22(tm2364) also suppresses the chemotaxis defect of the daf-18 mutants. (B) pe902, pe905, pe917, pe922, and tm2364 cause defects in attraction behavior to the ASER-sensed chemical ammonium chloride. (C) Genomic structure of gcy-22 receptor-type guanylyl cyclase. Solid boxes indicate predicted protein domains. Locations of the four suppressor mutations in the gcy-22 gene are depicted. Error bars represent standard error of the mean (SEM). (***) P < 0.001, (**) P < 0.01, Bonferroni t-test.