Table 1.

Parameters describing the energy barrier (x_u, k_u, ΔG_u^‡) and the mechanical spring constant (κ) of stable structural segments of bovine and mouse rhodopsin. Parameters computed from DFS plots, as described in Materials and Methods, are shown with the standard error.

Stable structural segments (peak position)	xu (nm) ± SE		ku (s−1) ± SE		ΔGu‡ ± SE		κ (N/m) ± SE
	Bovine	Mouse	Bovine	Mouse	Bovine	Mouse	Bovine	Mouse
N1 (15 aa)	0.57 ± 0.15	0.36 ± 0.14	0.03 ± 0.10	0.37 ± 1.27	24.21 ± 3.17	21.73 ± 3.47	0.62 ± 0.25	1.39 ± 0.85
N2 (26 aa)	0.36 ± 0.05	0.34 ± 0.10	0.46 ± 0.59	0.36 ± 0.89	21.51 ± 1.29	21.74 ± 2.46	1.36 ± 0.33	1.55 ± 0.70
H1-C1-H2 (37 aa)	0.36 ± 0.05	0.30 ± 0.08	0.36 ± 0.40	0.89 ± 1.80	21.74 ± 1.10	20.84 ± 2.02	1.41 ± 0.29	1.98 ± 0.88
E1 (97 aa)	0.40 ± 0.04	0.45 ± 0.08	0.12 ± 0.12	0.06 ± 0.11	22.81 ± 0.96	23.46 ± 1.63	1.21 ± 0.22	0.96 ± 0.27
H3-C2-H4-E2 (108 aa)	0.28 ± 0.04	0.28 ± 0.05	0.33 ± 0.39	0.38 ± 0.45	21.84 ± 1.20	21.70 ± 1.18	2.36 ± 0.60	2.22 ± 0.58
H5-C3-H6.1 (122 aa)	0.27 ± 0.03	0.37 ± 0.02	0.32 ± 0.32	0.03 ± 0.01	21.87 ± 0.99	24.18 ± 0.47	2.54 ± 0.55	1.44 ± 0.11
H6.2-E3-H7 (169 aa)	1.22 ± 0.38	0.62 ± 0.21	0.00 ± 0.00	0.15 ± 0.38	28.04 ± 4.18	22.60 ± 2.50	0.16 ± 0.08	0.48 ± 0.27
H8 (220 aa)	0.67 ± 0.18	0.59 ± 0.23	0.01 ± 0.03	0.02 ± 0.07	25.39 ± 2.75	24.71 ± 3.82	0.47 ± 0.20	0.58 ± 0.37
CT (238 aa)	0.35 ± 0.06	0.41 ± 0.12	0.33 ± 0.38	0.19 ± 0.38	21.83 ± 1.14	22.41 ± 2.04	1.52 ± 0.45	1.09 ± 0.52