TABLE 1 .
Soluble sugar production following digestion of hatched wheat straw (17-h incubation period) by various enzyme combinationsa
| Enzyme combination | Amt of sugar producedb |
||||
|---|---|---|---|---|---|
| Arabinose | Xylose | Xylobiose | Cellobiose | Xylotriose | |
| Wild-type free enzymes | 6.8 ± 0.1 (45.7) | 6.8 ± 0.3 (45.7) | 19 ± 0.6 (68.6) | 46.7 ± 1.4 (137.1) | 4.5 ± 0.4 (10.9) |
| Divalent cellulase- and xylanase-containing designer cellulosomes | 6.5 ± 0.5 (42.8) | 5.3 ± 0.3 (34.2) | 12.7 ± 1.5 (45.7) | 58.7 ± 1.9 (168.6) | 11.3 ± 0.7 (27.1) |
| Tetravalent cellulase/xylanase-containing designer cellulosome | 6.8 ± 0.2 (45.7) | 7.8 ± 0.1 (51.4) | 31.6 ± 0.7 (111.4) | 61.5 ± 1 (180) | 12.7 ± 0.6 (30.6) |
a
Data were obtained by HPLC analysis. The absence of glucose was confirmed by using a glucose assay kit. Values for xylose were corroborated using a xylose assay kit. An unidentified peak, present only after enzymatic treatments, eluted at ~3.9 min (between the xylose and xylobiose peaks) and was possibly a modified monosaccharide.
b
Values are shown in milligrams per gram of substrate (mean ± standard deviation). The values in parentheses are micromoles per gram of substrate.