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NTg, Tg-Sirt1 and cardiac specific Sirt1 -/- mice were subjected to I/R or sham operation. Twenty-four hours after reperfusion, the heart was subjected to immunostaining with anti-troponin T antibody (red), anti-FoxO1 antibody (green) and DAPI (blue). FoxO1 positive nuclei/total nuclei was evaluated and expressed as %.
