Table I.
Incorporation of [3H]mannose into [3H]Man-glycoprotein by metabolic labeling of various yeast strains
| Yeast strain | [3H-Man]glycoprotein
(dpm × 10 − 6/mg) |
|---|---|
| rer2Δ | 4.3 |
| rer2Δ/YEp352 | 8.6 |
| Wild type (SS328) | 42.6 |
| rer2Δ/UPPS | 26.9 |
| rer2Δ/RER2 | 32.7 |
Cells from the indicated yeast strains were labeled metabolically for 15 min with [3H]mannose and analyzed for incorporation of [3H]mannose into the glycoprotein fraction as described in “Materials and methods”. The data are average values of duplicate analyses and are representative of several experiments.