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Stress-induced Pabp granules colocalize with poly(A)+ RNA (A) but exclude Hsp104 (B). The distribution of poly(A)+ RNA after treatment with 1 M KCl for 30 min, or without glucose for 15 min, was examined in wt cells expressing Pabp-GFP or Hsp104-GFP. Poly(A)+ RNA was visualized by (dT)25-oligomers coupled to Alexa Fluor 568 (Invitrogen) (see Materials and Methods).
