Effects of eIF2α, Sty1, and Gcn2 on granule formation and disintegration after hyperosmotic shock. (A) Pabp-RFP localization after stress in wt cells, or strains lacking wt eIF2α, Sty1, or Gcn2. First row, wt; second row, eIF2α-S52A; third row, gcn2Δ; fourth row, sty1Δ. (B) Diagram representing the percentage, from each strain, of cells with ≥1 granules plotted against time after exposure to 1 M KCl.