FIGURE 6.

pka1Δ mutants are unable to form granules containing Pabp and down-regulate translation upon glucose deprivation, but do form granules in hyperosmotic stress. (A) The localization of Pabp-GFP or Dcp2-GFP in a pka1Δ background was examined after different types of stress treatment. (B) Polysomal profiles upon glucose starvation (15 min). Ribosomal material and polysomes were separated by sedimentation ultracentrifugation and detected online (see Materials and Methods). Left chromatogram, wt cells; right chromatogram, pka1Δ. Dashed line, no stress; solid line, −glucose.