FIGURE 7.

External CaCl₂ and staurosporine enhance granule formation upon glucose starvation. Wild-type cells expressing Pabp-RFP were resuspended in glucose-free medium, or glucose-free medium supplemented with 100 mM of CaCl₂ or 2 μg/mL staurosporine. (A) Left panel, glucose deprivation; middle panel, glucose deprivation + CaCl₂; right panel, glucose deprivation + staurosporine. (B) Graph depicting the number of granules per cell formed after each condition. RNA granules were quantified using Image J and Adobe Photoshop.