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. Author manuscript; available in PMC: 2011 Dec 21.
Published in final edited form as: Biochemistry. 2010 Nov 24;49(50):10623–10635. doi: 10.1021/bi101389r

Figure 2.

Figure 2

REDOR S0 and S1 13C SSNMR spectra at 32.2 ms dephasing time for (a) HFP-NC, (b) HFP-P, (c) HFP-A, or (d) HFP-AP. Each spectrum was processed with 200 Hz line broadening and baseline correction and was the sum of: (a) 38624; (b) 23488; (c) 24914; or (d) 14240 scans. Relatively narrow 13CO signals were observed in the HFP-P, HFP-A, and HFP-AP samples because the HFPs were membrane-associated with predominant β sheet conformation at the labeled 13CO site. A broader 13CO signal was observed in the HFP-NC sample because there was no membrane and there were populations of lyophilized HFP with either α helical or β sheet conformation at the labeled 13CO site.