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. 2010 Oct 7;60(1):157–167. doi: 10.2337/db10-0331

FIG. 2.

FIG. 2.

Markers of mitochondrial biogenesis after chronic exercise. A: Real-time PCR analyses of primary mitochondrial transcripts ND5 (complex I) and CYTB (complex III). B: Real-time PCR analyses of primary mitochondrial transcript cytochrome oxidase III (COX III; complex IV) and the gluconeogenic phosphoenolpyruvate carboxykinase (PEPCK) in skeletal muscle samples. All data are normalized per 18S rRNA. C: Citrate synthase enzyme activity (IU/g wet weight) and relative copy number of mtDNA per diploid nuclear genome in the deep-red portion of the gastrocnemius muscle. Numbers of animals are as described in Fig. 1. Data are mean ± SEM. *P < 0.05; **P < 0.01. C, controls; r.U., relative units; T, trained mice.