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SIRT1, phospho-AMPK, and phospho-ACC protein expression after chronic exercise. A: Densitometry of protein data. Homogenates of skeletal muscle probed with an antibody detecting SIRT1 (110 kDa), phospho-AMPK (62 kDa, Thr172), AMPK (62kDa), phospho-ACC (280 kDa, Ser79), ACC (280 kDa), and GAPDH (42 kDa) as a loading control. Data are means ± SEM. **P < 0.01. C, control animals; r.U., relative units; T, trained animals. B: Representative Western blots.
