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. 1995 Oct;6(10):1411–1421. doi: 10.1091/mbc.6.10.1411

RACH2, a novel human gene that complements a fission yeast cell cycle checkpoint mutation.

S Davey 1, D Beach 1
PMCID: PMC301296  PMID: 8573795

Abstract

We have identified a novel human gene by virtue of its ability to complement the rad1-1 checkpoint mutant of Schizosaccharomyces pombe. This gene, called RACH2, rescues the temperature-sensitive lethality of a rad1-1 wee1-50 double mutant of S. pombe. Expression of RACH2 in S. pombe rad1-1 strains partially restores UV resistance to the rad1-1 mutant strain. Expression of RACH2 in a rad1-1 cdc25-22 double mutant partially restores the dose-dependent delay in mitotic entry after irradiation that is lost in rad1-1 checkpoint-deficient mutants. Overexpression of RACH2 in human tissue culture cells induces apoptosis.

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