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. 2011 Jan;132(1):134–143. doi: 10.1111/j.1365-2567.2010.03348.x

Figure 1.

Figure 1

Pre-treatment of bone-marrow-derived dendritic cells (BMDC) inhibits lipopolysaccharide (LPS) -induced co-stimulatory molecule expression and cytokine production. Bone marrow cells grown in the presence of granulocyte–macrophage colony-stimulating factor (GM-CSF) for 5 days were harvested and treated with 50 μm GD1a in EHAA supplemented with 1% fetal calf serum. (a) After 24 hr the cells were stimulated with 200 ng/ml lipopolysaccharide (LPS) and then cells were harvested and protein expression on CD11c+ cells was analysed by flow cytometry. (b) Cells were prepared as above and supernatants from the BMDC treated with or without GD1a and LPS as indicated were analysed by ELISA. (c) BMDC prepared as above were treated with 50 μm GM1 for 24 hr and then exposed to various doses of LPS as indicated for an additional 24 hr. Supernatants were collected and analysed by ELISA. The ELISA data are an average (+ SD) obtained from two to four wells from two independent two-fold serial dilutions for each sample. *P < 0·05 for all groups compared with control. The results are representative of three independent experiments.