Figure 3.

Initial T-cell activation is not affected after priming with ganglioside-treated bone-marrow-derived dendritic cells (BMDC). B6 AND T-cell receptor T cells were stimulated with GD1a-treated or untreated control BMDC and pMCC as in Fig. 2 except in the absence of exogenous interleukin-2 (IL-2). (a) T cells were harvested 24 hr after priming and analysed for CD25 and CD69 expression after gating on CD4⁺ cells. (b) Before priming, T cells were loaded with 0·5 μm CFSE, stimulated as indicated, and then 3 and 4 days later were harvested and the degree of CFSE dye dilution was assessed on CD4⁺ T cells. These data are representative of three independent experiments.