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. 2011 Jan;132(1):134–143. doi: 10.1111/j.1365-2567.2010.03348.x

Figure 6.

Figure 6

Inhibitory effect of T cells primed with GD1a-treated bone-marrow-derived dendritic cells (BMDC) requires cell–cell contact. B6 AND T-cell receptor T cells were primed with GD1a-treated or untreated control BMDC and pMCC as in Fig. 2 for 5 days. Viable cells were harvested and re-stimulated in transwell culture dishes with 5 μg/ml pMCC. Wells contain T cells primed with control BMDC (control) and/or T cells primed with GD1a-treated BMDC (GD1a), as shown. Both the upper and lower wells in each condition (A–D) also contain 6 × 105 T-cell-depleted spleen cells as antigen-presenting cells. After 48 hr of culture, the supernatant was collected and analysed for interferon-γ (IFN-γ) by ELISA. Key: A, 3 × 105 T cells primed with control BMDC; B, 3 × 105 T cells primed with GD1a-treated BMDC; C, 3 × 105 T cells primed with control BMDC, mixed with 3 × 105 T cells primed with GD1a-treated BMDC; D, both as in C, but separated by the membrane. *P < 0·005 for all groups compared with control. These data are representative of two independent experiments.