Figure 3.

CD34⁺ CD45⁺ IL-5Rα⁺ cells increases in the airways and newly produced interleukin-5 receptor α subunit-positive (IL-5Rα⁺) lung cells proliferate after allergen challenge. (a) The number of CD34⁺ CD45⁺ IL-5Rα⁺ cells in the lung, analysed by FACS in ovalbumin (OVA) -sensitized/exposed BALB/c mice compared with OVA-sensitized but PBS-exposed mice. Open bars represent PBS-exposed animals and dark bar represents OVA-exposed animals. (b) Number of IL-5Rα⁺ BrdU⁺ 7-AAD⁺ cells in the lung, analysed by FACS in OVA-sensitized/exposed BALB/c mice compared with OVA-sensitized but PBS-exposed mice. Open bars represent cells gated on the SSC^high cell population and dark bars are cells gated on the SSC^low cell population. Data are shown as mean (+ SEM) (n = 5–6). *P< 0·05 and **P< 0·01 between OVA-exposed and PBS-exposed groups.