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. 1974 Sep;54(3):664–671. doi: 10.1172/JCI107804

Granulocyte Transfusion Therapy of Experimental Pseudomonas Pneumonia

David C Dale 1,2,3, Herbert Y Reynolds 1,2,3, James E Pennington 1,2,3, Ronald J Elin 1,2,3, Terry W Pitts 1,2,3, Robert G Graw Jr 1,2,3
PMCID: PMC301600  PMID: 4212040

Abstract

Pseudomonas pneumonia was produced in dogs with radiation-induced leukopenia. Treatment of this infection with either gentamicin alone or gentamicin plus daily granulocyte transfusion was compared in a randomized controlled trail. The dogs receiving granulocytes plus gentamicin survived significantly longer than those treated with gentamicin alone (P < 0.05). The Pseudomonas immunotype which was inoculated into the dogs were recovered at autopsy from none of the granulocyte-transfused dogs, whereas seven or eight of the dogs treated with gentamicin alone had the inoculated Pseudomonas immunotype in the area of induced pneumonia at autopsy. As measured by the limulus test, the granulocyte-transfused dogs also did not have endotoxemia as frequently as the dogs given only gentamicin (P < 0.05). This controlled study establishes that transfused granulocytes can favorably alter the course of experimental Pseudomonas pneumonia and suggests that granulocyte transfusion may be a useful therapy in serious bacterial infections of leukopenic subjects.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAUDE A. I., CAREY F. J., ZALESKY M. Studies with radioactive endotoxin. II. Correlation of physiologic effects with distribution of radioactivity in rabbits injected with radioactive sodium chromate. J Clin Invest. 1955 Jun;34(6):858–866. doi: 10.1172/JCI103141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRECHER G., WILBUR K. M., CRONKITE E. P. Transfusion of separated leukocytes into irradiated dogs with aplastic marrows. Proc Soc Exp Biol Med. 1953 Oct;84(1):54–56. doi: 10.3181/00379727-84-20539. [DOI] [PubMed] [Google Scholar]
  3. Benbunan M., Bussel A., Reviron J., Boiron M., Bernard J. Les transfusions de granulocytes normaux prélevés sur séparateur de cellules. Résultats techniques et cliniques. Nouv Rev Fr Hematol. 1973 Jul-Aug;13(4):469–485. [PubMed] [Google Scholar]
  4. Bodey G. P., Whitecar J. P., Jr, Middleman E., Rodriguez V. Carbenicillin therapy for pseudomonas infections. JAMA. 1971 Oct 4;218(1):62–66. [PubMed] [Google Scholar]
  5. Bona C. A. Fate of endotoxin in macrophages: biological and ultrastructural aspects. J Infect Dis. 1973 Jul;128(Suppl):74–81. doi: 10.1093/infdis/128.supplement_1.s74. [DOI] [PubMed] [Google Scholar]
  6. Buckner D., Eisel R., Perry S. Blood cell separation in the dog by continuous flow centrifugation. Blood. 1968 May;31(5):653–672. [PubMed] [Google Scholar]
  7. Burdi A. R., Garn S. M., Miller R. L., Nagy J. M. Sequence polymorphism in prenatal dental development. J Dent Res. 1970 Nov-Dec;49(6 Suppl):1554–1554. doi: 10.1177/00220345700490067201. [DOI] [PubMed] [Google Scholar]
  8. Chedid L., Lamensans A., Prixova J. Comparison of the effects of an antibacterial leukocytic extract and the serum endotoxin-detoxifying component of lipopolysaccharides extracted from rough and smooth Salmonellae. J Infect Dis. 1970 Jun;121(6):634–639. doi: 10.1093/infdis/121.6.634. [DOI] [PubMed] [Google Scholar]
  9. Elin R. J., Wolff S. M. Nonspecificity of the limulus amebocyte lysate test: positive reactions with polynucleotides and proteins. J Infect Dis. 1973 Sep;128(3):349–352. doi: 10.1093/infdis/128.3.349. [DOI] [PubMed] [Google Scholar]
  10. Epstein R. B., Clift R. A., Thomas E. D. The effect of leukocyte transfusions on experimental bacteremia in the dog. Blood. 1969 Dec;34(6):782–790. [PubMed] [Google Scholar]
  11. Epstein R. B., Waxman F. J., Bennett B. T., Andersen B. R. Pseudomonas septicemia in neutropenic dogs. I. Treatment with granulocyte transfusions. Transfusion. 1974 Jan-Feb;14(1):51–57. doi: 10.1111/j.1537-2995.1974.tb04484.x. [DOI] [PubMed] [Google Scholar]
  12. Eyre H. J., Goldstein I. M., Perry S., Graw R. G., Jr Leukocyte transfusions: function of transfused granulocytes from donors with chronic myelocytic leukemia. Blood. 1970 Oct;36(4):432–442. [PubMed] [Google Scholar]
  13. FREIREICH E. J., LEVIN R. H., WHANG J., CARBONE P. P., BRONSON W., MORSE E. E. THE FUNCTION AND FATE OF TRANSFUSED LEUKOCYTES FROM DONORS WITH CHRONIC MYELOCYTIC LEUKEMIA IN LEUKOPENIC RECIPIENTS. Ann N Y Acad Sci. 1964 Feb 28;113:1081–1089. doi: 10.1111/j.1749-6632.1964.tb40726.x. [DOI] [PubMed] [Google Scholar]
  14. Graw R. G., Jr, Herzig G. P., Eisel R. J., Perry S. Leukocyte and platelet collection from normal donors with the continuous flow blood cell separator. Transfusion. 1971 Mar-Apr;11(2):94–101. doi: 10.1111/j.1537-2995.1971.tb04383.x. [DOI] [PubMed] [Google Scholar]
  15. Graw R. G., Jr, Herzig G., Perry S., Henderson E. S. Normal granulocyte transfusion therapy: treatment of septicemia due to gram-negative bacteria. N Engl J Med. 1972 Aug 24;287(8):367–371. doi: 10.1056/NEJM197208242870801. [DOI] [PubMed] [Google Scholar]
  16. Levin R. H., Whang J., Carbone P. P., Freireich E. J. Erythroid homograft following leukocyte transfusion in a patient with acute leukemia. I. Clinical studies and implications. Blood. 1965 Nov;26(5):587–596. [PubMed] [Google Scholar]
  17. Levine A. S., Graw R. G., Jr, Young R. C. Management of infections in patients with leukemia and lymphoma: current concepts and experimental approaches. Semin Hematol. 1972 Apr;9(2):141–179. [PubMed] [Google Scholar]
  18. McCredie K. B., Freireich E. J., Hester J. P., Vallejos C. Leukocyte transfusion therapy for patients with host-defense failure. Transplant Proc. 1973 Sep;5(3):1285–1289. [PubMed] [Google Scholar]
  19. Moreau S. C., Skarnes R. C. Host resistance to bacterial endotoxemia: mechanisms in endotoxin-tolerant animals. J Infect Dis. 1973 Jul;128(Suppl):122–133. doi: 10.1093/infdis/128.supplement_1.s122. [DOI] [PubMed] [Google Scholar]
  20. Pennington J. E., Reynolds H. Y., Carbone P. P. Pseudomonas pneumonia. A retrospective study of 36 cases. Am J Med. 1973 Aug;55(2):155–160. doi: 10.1016/0002-9343(73)90163-0. [DOI] [PubMed] [Google Scholar]
  21. Pennington J. E., Reynolds H. Y. Concentrations of gentamicin and carbenicillin in bronchial secretions. J Infect Dis. 1973 Jul;128(1):63–68. doi: 10.1093/infdis/128.1.63. [DOI] [PubMed] [Google Scholar]
  22. RAAB S. O., ATHENS J. W., HAAB O. P., BOGGS D. R., ASHENBRUCKER H., CARTWRIGHT G. E., WINTROBE M. M. GRANULOKINETICS IN NORMAL DOGS. Am J Physiol. 1964 Jan;206:83–88. doi: 10.1152/ajplegacy.1964.206.1.83. [DOI] [PubMed] [Google Scholar]
  23. Reynolds H. Y., Thompson R. E. Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with pseudomonas aeruginosa. J Immunol. 1973 Aug;111(2):358–368. [PubMed] [Google Scholar]
  24. Schimpff S., Satterlee W., Young V. M., Serpick A. Empiric therapy with carbenicillin and gentamicin for febrile patients with cancer and granulocytopenia. N Engl J Med. 1971 May 13;284(19):1061–1065. doi: 10.1056/NEJM197105132841904. [DOI] [PubMed] [Google Scholar]
  25. Schwarzenberg L., Mathé G., de Grouchy J., de Nava C., de Vries M. J., Amiel J. L., Cattan A., Schneider M., Schlumberger J. R. White blood cell transfusions. Isr J Med Sci. 1965 Sep;1(5):925–956. [PubMed] [Google Scholar]
  26. Tattersall M. H., Spiers A. S., Darrell J. H. Initial therapy with combination of five antibiotics in febrile patients with leukaemia and neutropenia. Lancet. 1972 Jan 22;1(7743):162–165. doi: 10.1016/s0140-6736(72)90567-3. [DOI] [PubMed] [Google Scholar]

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