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. 1974 Sep;54(3):710–717. doi: 10.1172/JCI107809

Identification of an Intestinal Sodium and Calcium-Dependent Phosphatase Stimulated by Parathyroid Hormone

Stanley J Birge 1,2, Helen R Gilbert 1,2
PMCID: PMC301605  PMID: 4852407

Abstract

Previous reports suggest that the site of the energy-dependent intestinal calcium transport against an electropotential and concentration gradient is located along the basal-lateral membrane of the mucosal cell. Accordingly, basal-lateral membranes were prepared from rat intestinal homogenates in order to identify the enzyme mediating this step in the transport process. An alkaline phosphatase was delineated which utilized ATP as a substrate and was dependent on both Na- and Ca++ with optimum enzyme activity at 200 mM and 0.04 mM, respectively. Furthermore, the activity of the enzyme was demonstrated to decrease with the advance in age of the animal and to decrease with removal of the parathyroid glands, consistent with a decreased rate of 45Ca release from mucosal cells under the same experimental conditions. Calcium binding to basal-lateral membrane fragments was also sodium dependent and enhanced by the prior administration of parathyroid extract. The consistent correlation between the rate of calcium transport across the basal-lateral membrane of the mucosal cell and the activity of this Na, Ca-dependent phosphatase under a variety of experimental conditions suggest that this enzyme may mediate the parathyroid hormone-sensitive active transport of calcium across the intestine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRESCIANI F., AURICCHIO F. Subcellular distribution of some metallic cations in the early stages of liver carcinogenesis. Cancer Res. 1962 Dec;22:1284–1289. [PubMed] [Google Scholar]
  2. Baker P. F. Transport and metabolism of calcium ions in nerve. Prog Biophys Mol Biol. 1972;24:177–223. doi: 10.1016/0079-6107(72)90007-7. [DOI] [PubMed] [Google Scholar]
  3. Birge S. J., Jr, Gilbert H. R., Avioli L. V. Intestinal calcium transport: the role of sodium. Science. 1972 Apr 14;176(4031):168–170. doi: 10.1126/science.176.4031.168. [DOI] [PubMed] [Google Scholar]
  4. Birge S. J., Switzer S. C., Leonard D. R. Influence of sodium and parathyroid hormone on calcium release from intestinal mucosal cells. J Clin Invest. 1974 Sep;54(3):702–709. doi: 10.1172/JCI107808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Borle A. B. Calcium metabolism in HeLa cells and the effects of parathyroid hormone. J Cell Biol. 1968 Mar;36(3):567–582. doi: 10.1083/jcb.36.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cha Y. N., Shin B. C., Lee K. S. Active uptake of Ca++ and Ca plus,plus-activated Mg++ ATPase in red cell membrane fragments. J Gen Physiol. 1971 Feb;57(2):202–215. doi: 10.1085/jgp.57.2.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chang C. H., Moog F. Alkaline phosphatases of the chicken duodenum. I. Isolation and partial characterization of the multiple forms of duodenal phosphatase in pre- and post-hatching stages. Biochim Biophys Acta. 1972 Jan 20;258(1):154–165. doi: 10.1016/0005-2744(72)90975-8. [DOI] [PubMed] [Google Scholar]
  8. DAHLQVIST A. METHOD FOR ASSAY OF INTESTINAL DISACCHARIDASES. Anal Biochem. 1964 Jan;7:18–25. doi: 10.1016/0003-2697(64)90115-0. [DOI] [PubMed] [Google Scholar]
  9. Forstner G. G., Sabesin S. M., Isselbacher K. J. Rat intestinal microvillus membranes. Purification and biochemical characterization. Biochem J. 1968 Jan;106(2):381–390. doi: 10.1042/bj1060381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fujita M., Ota H., Kawai K., Matsui H., Nakao M. Differential isolation of microvillous and basolateral plasma membranes from intestinal mucosa: mutually exclusive distribution of digestive enzymes and ouabain-sensitive ATPase. Biochim Biophys Acta. 1972 Aug 9;274(2):336–347. doi: 10.1016/0005-2736(72)90181-2. [DOI] [PubMed] [Google Scholar]
  11. Ingersoll R. J., Wasserman R. H. Vitamin D3-induced calcium-binding protein. Binding characteristics, conformational effects, and other properties. J Biol Chem. 1971 May 10;246(9):2808–2814. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Lifshitz F., Harrison H. C., Harrison H. E. Influence of parathyroid function upon the in vitro transport of calcium and phosphate by the rat intestine. Endocrinology. 1969 Apr;84(4):912–917. doi: 10.1210/endo-84-4-912. [DOI] [PubMed] [Google Scholar]
  14. Makinose M., Hasselbach W. ATP synthesis by the reverse of the sarcoplasmic calcium pump. FEBS Lett. 1971 Jan 30;12(5):271–272. doi: 10.1016/0014-5793(71)80196-5. [DOI] [PubMed] [Google Scholar]
  15. Martin D. L., DeLuca H. F. Influence of sodium on calcium transport by the rat small intestine. Am J Physiol. 1969 Jun;216(6):1351–1359. doi: 10.1152/ajplegacy.1969.216.6.1351. [DOI] [PubMed] [Google Scholar]
  16. Martin D. L., Melancon M. J., Jr, DeLuca H. F. Vitamin D stimulated, calcium-dependent adenosine triphosphatase from brush borders of rat small intestine. Biochem Biophys Res Commun. 1969 Jun 27;35(6):819–823. doi: 10.1016/0006-291x(69)90697-4. [DOI] [PubMed] [Google Scholar]
  17. Moog F., Glazier H. S. Phosphate absorption and alkaline phosphatase activity in the small intestine of the adult mouse and of the chick embryo and hatched chick. Comp Biochem Physiol A Comp Physiol. 1972 Jun 1;42(2):321–336. doi: 10.1016/0300-9629(72)90113-2. [DOI] [PubMed] [Google Scholar]
  18. Oschman J. L., Wall B. J. Calcium binding to intestinal membranes. J Cell Biol. 1972 Oct;55(1):58–73. doi: 10.1083/jcb.55.1.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Quigley J. P., Gotterer G. S. Distribution of (Na+-K+)-stimulated ATPase activity in rat intestinal mucosa. Biochim Biophys Acta. 1969 Apr;173(3):456–468. doi: 10.1016/0005-2736(69)90010-8. [DOI] [PubMed] [Google Scholar]
  20. RASMUSSEN H. The influence of parathyroid function upon the transport of calcium in isolated sacs of rat small intestine. Endocrinology. 1959 Sep;65:517–519. doi: 10.1210/endo-65-3-517. [DOI] [PubMed] [Google Scholar]
  21. SCHACHTER D., DOWDLE E. B., SCHENKER H. Active transport of calcium by the small intestine of the rat. Am J Physiol. 1960 Feb;198:263–268. doi: 10.1152/ajplegacy.1960.198.2.263. [DOI] [PubMed] [Google Scholar]
  22. SKOU J. C. ENZYMATIC BASIS FOR ACTIVE TRANSPORT OF NA+ AND K+ ACROSS CELL MEMBRANE. Physiol Rev. 1965 Jul;45:596–617. doi: 10.1152/physrev.1965.45.3.596. [DOI] [PubMed] [Google Scholar]
  23. SMITH L. Spectrophotometric assay of cytochrome c oxidase. Methods Biochem Anal. 1955;2:427–434. doi: 10.1002/9780470110188.ch13. [DOI] [PubMed] [Google Scholar]
  24. Saini P. K., Done J. The diversity of alkaline phosphatase from rat intestine. Isolation and purification of the enzyme (s). Biochim Biophys Acta. 1972 Jan 20;258(1):147–153. doi: 10.1016/0005-2744(72)90974-6. [DOI] [PubMed] [Google Scholar]
  25. Schachter D., Kowarski S., Finkelstein J. D., Ma R. I. Tissue concentration differences during active transport of calcium by intestine. Am J Physiol. 1966 Nov;211(5):1131–1136. doi: 10.1152/ajplegacy.1966.211.5.1131. [DOI] [PubMed] [Google Scholar]
  26. Schatzmann H. J., Vincenzi F. F. Calcium movements across the membrane of human red cells. J Physiol. 1969 Apr;201(2):369–395. doi: 10.1113/jphysiol.1969.sp008761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wallach S., Reizenstein D. L., Bellavia J. V. The cellular transport of calcium in rat liver. J Gen Physiol. 1966 Mar;49(4):743–762. doi: 10.1085/jgp.49.4.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Walling M. W., Rothman S. S. Phosphate-independent, carrier-mediated active transport of calcium by rat intestine. Am J Physiol. 1969 Oct;217(4):1144–1148. doi: 10.1152/ajplegacy.1969.217.4.1144. [DOI] [PubMed] [Google Scholar]
  29. Wasserman R. H., Taylor A. N. Vitamin d3-induced calcium-binding protein in chick intestinal mucosa. Science. 1966 May 6;152(3723):791–793. doi: 10.1126/science.152.3723.791. [DOI] [PubMed] [Google Scholar]

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